Cloning of parsley flavone synthase I

Stefan Martens; Gert Forkmann; Ulrich Matern; Richard Lukačin

doi:10.1016/S0031-9422(01)00191-1

Cloning of parsley flavone synthase I

Stefan Martens
, Gert Forkmann
, Ulrich Matern
, Richard Lukačin

Life Sciences

Technical University of Munich
Philipps-Universität Marburg

Research output: Contribution to journal › Article › peer-review

109 Scopus citations

Abstract

A cDNA encoding flavone synthase I was amplified by RT-PCR from leaflets of Petroselinum crispum cv. Italian Giant seedlings and functionally expressed in yeast cells. The identity of the recombinant, 2-oxoglutarate-dependent enzyme was verified in assays converting (2S)-naringenin to apigenin.

Original language	English
Pages (from-to)	43-46
Number of pages	4
Journal	Phytochemistry
Volume	58
Issue number	1
DOIs	https://doi.org/10.1016/S0031-9422(01)00191-1
State	Published - 10 Aug 2001

Keywords

2-Oxoglutarate-dependent dioxygenase
Apiaceae
Flavone synthase I cloning
Flavonoid biosynthesis
Heterologous expression
Petroselinum crispum

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10.1016/S0031-9422(01)00191-1

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title = "Cloning of parsley flavone synthase I",

abstract = "A cDNA encoding flavone synthase I was amplified by RT-PCR from leaflets of Petroselinum crispum cv. Italian Giant seedlings and functionally expressed in yeast cells. The identity of the recombinant, 2-oxoglutarate-dependent enzyme was verified in assays converting (2S)-naringenin to apigenin.",

keywords = "2-Oxoglutarate-dependent dioxygenase, Apiaceae, Flavone synthase I cloning, Flavonoid biosynthesis, Heterologous expression, Petroselinum crispum",

author = "Stefan Martens and Gert Forkmann and Ulrich Matern and Richard Luka{\v c}in",

year = "2001",

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T1 - Cloning of parsley flavone synthase I

AU - Martens, Stefan

AU - Forkmann, Gert

AU - Matern, Ulrich

AU - Lukačin, Richard

PY - 2001/8/10

Y1 - 2001/8/10

N2 - A cDNA encoding flavone synthase I was amplified by RT-PCR from leaflets of Petroselinum crispum cv. Italian Giant seedlings and functionally expressed in yeast cells. The identity of the recombinant, 2-oxoglutarate-dependent enzyme was verified in assays converting (2S)-naringenin to apigenin.

AB - A cDNA encoding flavone synthase I was amplified by RT-PCR from leaflets of Petroselinum crispum cv. Italian Giant seedlings and functionally expressed in yeast cells. The identity of the recombinant, 2-oxoglutarate-dependent enzyme was verified in assays converting (2S)-naringenin to apigenin.

KW - 2-Oxoglutarate-dependent dioxygenase

KW - Apiaceae

KW - Flavone synthase I cloning

KW - Flavonoid biosynthesis

KW - Heterologous expression

KW - Petroselinum crispum

UR - https://www.scopus.com/pages/publications/0035839112

U2 - 10.1016/S0031-9422(01)00191-1

DO - 10.1016/S0031-9422(01)00191-1

M3 - Article

C2 - 11524111

AN - SCOPUS:0035839112

SN - 0031-9422

VL - 58

SP - 43

EP - 46

JO - Phytochemistry

JF - Phytochemistry

IS - 1

ER -

Cloning of parsley flavone synthase I

Abstract

Keywords

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