Cloning, expression and purification of the human Islet Amyloid Polypeptide (hIAPP) from Escherichia coli

Keyphrases

• Escherichia Coli 100%
• Cloning Expression 100%
• Human Islet Amyloid Polypeptide (hIAPP) 100%
• N-terminus 40%
• Chitin-binding Domain 40%
• Hydrogen Peroxide 20%
• Solid State 20%
• Small Molecules 20%
• Type 2 Diabetes Mellitus (T2DM) 20%
• Purification Process 20%
• C-terminus 20%
• Cell Toxicity 20%
• Disulfide Bond 20%
• Protein Aggregates 20%
• Pancreatic Cells 20%
• Amyloid Fibrils 20%
• Minimal Media 20%
• Cell Loss 20%
• Amyloid Aggregates 20%
• Native Peptides 20%
• Amidation 20%
• Human IAPP 20%
• V8 Protease 20%
• LB Medium 20%
• Intein Splicing 20%

Immunology and Microbiology

• Chitin Binding 100%
• Escherichia coli 100%
• Amino Terminal Sequence 100%
• Amidation 50%
• Solution and Solubility 50%
• Fibril 50%
• Carboxy Terminal Sequence 50%
• Cytotoxicity 50%
• Hydrogen Peroxide 50%
• Cell Loss 50%
• Disulfide Bond 50%
• Solubilization 50%

Biochemistry, Genetics and Molecular Biology

• Copurification 100%
• Escherichia coli 100%
• Amylin 100%
• N-Terminus 33%
• Binding Domain 33%
• Chitin Binding 33%
• Solution and Solubility 16%
• Small Molecule 16%
• Disulfide Bond 16%
• C-Terminus 16%
• Hydrogen Peroxide 16%
• Cell Loss 16%
• Amidation 16%
• Intein 16%
• Cytotoxicity 16%
• Protease 16%
• Solubilization 16%
• Amyloid Protein 16%