Cloning, bacterial synthesis, and characterization of immunoglobulin variable regions of a monoclonal antibody specific for the hepatitis B virus X protein

Jasper Zu Putlitz; Arne Skerra; Claus Hobe Schröder; Hanswalter Zentgraf; Jack R. Wands

doi:10.1016/S0378-1119(98)00419-3

Cloning, bacterial synthesis, and characterization of immunoglobulin variable regions of a monoclonal antibody specific for the hepatitis B virus X protein

Jasper Zu Putlitz
, Arne Skerra
, Claus Hobe Schröder
, Hanswalter Zentgraf
, Jack R. Wands

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The nucleotide (nt) sequences encoding the variable regions of the heavy (H) and light (L) chains were determined for a murine monoclonal antibody, 12/231/93, which is specific for a linear epitope located between amino acids 90 and 102 of the hepatitis B virus (HBV) X protein (HBx). The variable (v) regions of the H and L chains were shown to belong to the mouse H chain subgroup II (C) and κ L chain group III, respectively. The cloned variable region sequences were used for the production of a Fab fragment in Escherichia coli, which had binding activity for membrane immobilized recombinant HBx. These gene sequences may be useful for the study of HBx function in cells that will support HBV replication.

Original language	English
Pages (from-to)	143-149
Number of pages	7
Journal	Gene
Volume	221
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1119(98)00419-3
State	Published - 9 Oct 1998
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

DNA sequencing
HBV
Immunoglobulin
Mouse
Polymerase chain reaction
Recombinant DNA

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10.1016/S0378-1119(98)00419-3

Cite this

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title = "Cloning, bacterial synthesis, and characterization of immunoglobulin variable regions of a monoclonal antibody specific for the hepatitis B virus X protein",

abstract = "The nucleotide (nt) sequences encoding the variable regions of the heavy (H) and light (L) chains were determined for a murine monoclonal antibody, 12/231/93, which is specific for a linear epitope located between amino acids 90 and 102 of the hepatitis B virus (HBV) X protein (HBx). The variable (v) regions of the H and L chains were shown to belong to the mouse H chain subgroup II (C) and κ L chain group III, respectively. The cloned variable region sequences were used for the production of a Fab fragment in Escherichia coli, which had binding activity for membrane immobilized recombinant HBx. These gene sequences may be useful for the study of HBx function in cells that will support HBV replication.",

keywords = "DNA sequencing, HBV, Immunoglobulin, Mouse, Polymerase chain reaction, Recombinant DNA",

author = "\{Zu Putlitz\}, Jasper and Arne Skerra and Schr{\"o}der, \{Claus Hobe\} and Hanswalter Zentgraf and Wands, \{Jack R.\}",

note = "Funding Information: The authors would like to thank Dr Stefan Urban, Zentrum f{\"u}r Molekulare Biologie, Heidelberg, for his generous gift of recombinant HBx protein. This work was supported by Grants CA-35711 and AA-08169 from the National Institutes of Health. J.z.P. is on leave from the Department of Internal Medicine II, University Hospital Freiburg, Germany, and is supported by the Stipendienprogramm `Infektionsforschung' of the German Cancer Research Center, Heidelberg, Germany.",

year = "1998",

month = oct,

day = "9",

doi = "10.1016/S0378-1119(98)00419-3",

language = "English",

volume = "221",

pages = "143--149",

journal = "Gene",

issn = "0378-1119",

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T1 - Cloning, bacterial synthesis, and characterization of immunoglobulin variable regions of a monoclonal antibody specific for the hepatitis B virus X protein

AU - Zu Putlitz, Jasper

AU - Skerra, Arne

AU - Schröder, Claus Hobe

AU - Zentgraf, Hanswalter

AU - Wands, Jack R.

N1 - Funding Information: The authors would like to thank Dr Stefan Urban, Zentrum für Molekulare Biologie, Heidelberg, for his generous gift of recombinant HBx protein. This work was supported by Grants CA-35711 and AA-08169 from the National Institutes of Health. J.z.P. is on leave from the Department of Internal Medicine II, University Hospital Freiburg, Germany, and is supported by the Stipendienprogramm `Infektionsforschung' of the German Cancer Research Center, Heidelberg, Germany.

PY - 1998/10/9

Y1 - 1998/10/9

N2 - The nucleotide (nt) sequences encoding the variable regions of the heavy (H) and light (L) chains were determined for a murine monoclonal antibody, 12/231/93, which is specific for a linear epitope located between amino acids 90 and 102 of the hepatitis B virus (HBV) X protein (HBx). The variable (v) regions of the H and L chains were shown to belong to the mouse H chain subgroup II (C) and κ L chain group III, respectively. The cloned variable region sequences were used for the production of a Fab fragment in Escherichia coli, which had binding activity for membrane immobilized recombinant HBx. These gene sequences may be useful for the study of HBx function in cells that will support HBV replication.

AB - The nucleotide (nt) sequences encoding the variable regions of the heavy (H) and light (L) chains were determined for a murine monoclonal antibody, 12/231/93, which is specific for a linear epitope located between amino acids 90 and 102 of the hepatitis B virus (HBV) X protein (HBx). The variable (v) regions of the H and L chains were shown to belong to the mouse H chain subgroup II (C) and κ L chain group III, respectively. The cloned variable region sequences were used for the production of a Fab fragment in Escherichia coli, which had binding activity for membrane immobilized recombinant HBx. These gene sequences may be useful for the study of HBx function in cells that will support HBV replication.

KW - DNA sequencing

KW - HBV

KW - Immunoglobulin

KW - Mouse

KW - Polymerase chain reaction

KW - Recombinant DNA

UR - https://www.scopus.com/pages/publications/0032500889

U2 - 10.1016/S0378-1119(98)00419-3

DO - 10.1016/S0378-1119(98)00419-3

M3 - Article

C2 - 9852959

AN - SCOPUS:0032500889

SN - 0378-1119

VL - 221

SP - 143

EP - 149

JO - Gene

JF - Gene

IS - 1

ER -

Cloning, bacterial synthesis, and characterization of immunoglobulin variable regions of a monoclonal antibody specific for the hepatitis B virus X protein

Abstract

UN SDGs

Keywords

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