Cloned human and murine serotonin3A receptors expressed in human embryonic kidney 293 cells display different single-channel kinetics

Gerhard Hapfelmeier, Rainer Haseneder, Katrin Lampadius, Gerhard Rammes, Rainer Rupprecht, Walter Zieglgänsberger

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

In the present study, well-resolved single-channel events of cloned human and murine homomeric 5-hydroxytryptamine type 3 (h5-HT3A and m5-HT3A) receptors, expressed in human embryonic kidney 293 cells, are reported for the first time. 5-HT (1 μM) applied in Ca2+- and Mg2+-free external solution to excised outside-out membrane patches induced non-rectifying single-channel inward currents. These currents were not observed in untransfected cells or in the presence of the antagonist ondansetron (1 μM). The mean single-channel conductance of the h5-HT3A and m5-HT3A receptors was 48±8 pS and 46±7 pS, the mean reversal potential was 10.3±1.8 mV and 4.7±5.3 mV, respectively. The analysis of single-channel open-times revealed for both h5-HT3A and m5-HT3A receptors only one type of open state, but different mean open-times (7.1±2.9 ms vs. 4.1±0.8 ms) indicating species-dependent gating mechanisms of 5-HT3 receptors.

Original languageEnglish
Pages (from-to)44-48
Number of pages5
JournalNeuroscience Letters
Volume335
Issue number1
DOIs
StatePublished - 19 Dec 2002

Keywords

  • Patch-clamp
  • Serotonin type 3 receptor
  • Single-channel kinetics

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