Clinical value of protein expression of kallikrein-related peptidase 7 (KLK7) in ovarian cancer

Julia Dorn, Apostolos Gkazepis, Matthias Kotzsch, Marcus Kremer, Corinna Propping, Katharina Mayer, Karin Mengele, Eleftherios P. Diamandis, Marion Kiechle, Viktor Magdolen, Manfred Schmitt

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Expression of the kallikrein-related peptidase 7 (KLK7) is dysregulated in ovarian cancer. We assessed KLK7 expression by ELISA and quantitative immunohistochemistry and analyzed its association with clinicopathological parameters and patients' outcome. KLK7 antigen concentrations were determined in tumor tissue extracts of 98 ovarian cancer patients by ELISA. For analysis of KLK7 immunoexpression in ovarian cancer tissue microarrays, a manual quantitative scoring system as well as a software tool for quantitative high-throughput automated image analysis was used. In immunohistochemical analyses, expression levels of KLK7 were not associated with patients' outcome. However, in multivariate analyses, KLK7 antigen levels in tumor tissue extracts were significantly associated with both overall and progression-free survival: ovarian cancer patients with high KLK7 levels had a significantly, 2-fold lower risk of death [hazard ratio (HR)=0.51, 95% confidence interval (CI)=0.29-0.90, p=0.019] or relapse [HR=0.47, 95% CI=0.25-0.91, p=0.024), as compared with patients who displayed low KLK7 levels. Our results indicate that - in contrast to earlier findings - high KLK7 antigen levels in tumor tissue extracts may be associated with a better prognosis of ovarian cancer patients.

Original languageEnglish
Pages (from-to)95-107
Number of pages13
JournalBiological Chemistry
Volume395
Issue number1
DOIs
StatePublished - 1 Jan 2014
Externally publishedYes

Keywords

  • ELISA
  • immunohistochemistry
  • kallikrein-related peptidases
  • prognosis
  • tumor tissue

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