Class Switch Recombination Defects: impact on B cell maturation and antibody responses

Ellen D. Renner, Carolin E. Krätz, Jordan S. Orange, Beate Hagl, Stacey Rylaarsdam, Gundula Notheis, Anne Durandy, Troy R. Torgerson, Hans D. Ochs

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

To assess how B cell phenotype analysis correlates with antigen responses in patients with class switch recombination defects (CSRD) we quantified memory B cells by flow-cytometry and immunized CSRD patients with the neoantigen bacteriophage phiX174 (phage). CSRD patients showed uniformly absent or markedly reduced switched memory B cells (IgMIgDCD27+). CD40L patients had reduced CD27+ memory B cells (both non-switched and switched). In NEMO patients, results varied depending on the IKKγ gene variant. Three of four AID patients had normal percentages of CD27+ memory B cells while CD27+IgMIgD switched memory B cells were markedly reduced in all AID patients. Antibody response to phage was remarkably decreased with lack of memory amplification and class-switching in immunized CD40L, UNG deficient, and NEMO patients. Distinct B-cell phenotype pattern correlated with abnormal antibody responses to a T-cell dependent neoantigen, representing a powerful tool to identify CSRD patients.

Original languageEnglish
Article number108638
JournalClinical Immunology
Volume222
DOIs
StatePublished - Jan 2021

Keywords

  • B cell development
  • Class switch recombination defect (CSRD)
  • Clinical immunology
  • Hyper-IgM syndromes (HIGM)
  • Immunodeficiencies

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