Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.). 9. Gene MlZec1 from the Triticum dicoccoides-derived wheat line Zecoi-1

Volker Mohler, Friedrich J. Zeller, Gerhard Wenzel, Sai L.K. Hsam

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Abstract

The Triticum dicoccoides-derived wheat line Zecoi-1 provides effective protection against powdery mildew. F3 segregation analysis of Chinese Spring x Zecoi-1 hybrids showed that resistance in line Zecoi-1 is controlled by a single dominant gene. Amplified fragment length polymorphism (AFLP) analysis of bulked segregants from F3s showing the homozygous resistant and susceptible phenotypes identified eight markers, of which four were associated with the resistance allele in repulsion phase. Following the assignment of these four repulsion phase AFLP markers to wheat chromosome 2B with the aid of Chinese Spring nulli-tetrasomic lines, they were physically mapped in the terminal breakpoint interval 0.89 (2BL-6)-1.00 (telomere) of chromosome 2BL. Genetic and physical mapping of simple sequence repeat markers from the distal half of chromosome 2BL located the wild emmer-derived powdery mildew resistance gene distal of breakpoint 0.89 in deletion line 2BL-6. Based on disease response patterns, genomic origin and chromosomal location the resistance gene in Zecoi-1 is temporarily designated MlZec1.

Original languageEnglish
Pages (from-to)161-167
Number of pages7
JournalEuphytica
Volume142
Issue number1-2
DOIs
StatePublished - Jan 2005

Keywords

  • Breakpoint interval (bin) mapping
  • Genetic mapping
  • Molecular markers
  • Powdery mildew
  • Resistance genes
  • Wheat

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