Chemische Untersuchungen am Phagenreceptor von Staphylococcus epidermidis

Translated title of the contribution: Chemical studies on the phage receptor of Staphylococcus epidermidis

Karl Heinz Schleifer, Josef Steber

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Four different phages of Staphylococcus epidermidis showed an excellent adsorption on S. epidermidis cell walls which were purified by treatment with trypsin. No adsorption was found on isolated peptidoglycan or cell wall teichoic acid preparations. The O-acetyl groups occurring in the peptidoglycan have, in contrast to that of S. aureus, no influence on the phage receptor activity. The glucose substituents of the cell wall teichoic acid, on the other hand, are crucical for the phage adsorption. Chemical or enzymatic removal of a part of the glucose residues led to a reduced phage adsorption. After complete removal of the glucose residues the phage receptor activity of the cell walls was totally lost. This indicates that the glucose residues are required for phage attachment. A direct receptor function of glucose, however, was not demonstrable. It may be supposed that the glucose residues guarantee the maintenance of a defined arrangement of molecules or charge on the surface of the cell wall for the specific attachment of the phages. Studies with cell walls of various coagulase-negative and coagulase-positive strains have shown that all studied phages adsorb only on cell walls of S. epidermidis.

Translated title of the contributionChemical studies on the phage receptor of Staphylococcus epidermidis
Original languageGerman
Pages (from-to)251-270
Number of pages20
JournalArchives of Microbiology
Volume98
Issue number1
DOIs
StatePublished - Jan 1974
Externally publishedYes

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