Characterizing ATP processing by the AAA+ protein p97 at the atomic level

Mikhail Shein, Manuel Hitzenberger, Tat Cheung Cheng, Smruti R. Rout, Kira D. Leitl, Yusuke Sato, Martin Zacharias, Eri Sakata, Anne K. Schütz

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The human enzyme p97 regulates various cellular pathways by unfolding hundreds of protein substrates in an ATP-dependent manner, making it an essential component of protein homeostasis and an impactful pharmacological target. The hexameric complex undergoes substantial conformational changes throughout its catalytic cycle. Here we elucidate the molecular motions that occur at the active site in the temporal window immediately before and after ATP hydrolysis by merging cryo-EM, NMR spectroscopy and molecular dynamics simulations. p97 populates a metastable reaction intermediate, the ADP·Pi state, which is poised between hydrolysis and product release. Detailed snapshots reveal that the active site is finely tuned to trap and eventually discharge the cleaved phosphate. Signalling pathways originating at the active site coordinate the action of the hexamer subunits and couple hydrolysis with allosteric conformational changes. Our multidisciplinary approach enables a glimpse into the sophisticated spatial and temporal orchestration of ATP handling by a prototype AAA+ protein. (Figure presented.).

Original languageEnglish
Pages (from-to)363-372
Number of pages10
JournalNature Chemistry
Volume16
Issue number3
DOIs
StatePublished - Mar 2024
Externally publishedYes

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