Characterization of two β-glucosidase genes from Clostridiumthermocellum

Folke Gräbnitz; Walter L. Staudenbauer

doi:10.1007/BF01024629

Characterization of two β-glucosidase genes from Clostridiumthermocellum

Folke Gräbnitz
, Walter L. Staudenbauer

Chair of Microbiology

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Two β-glucosidase genes, designated bglA and bglB, were isolated from a gene bank of Clostridiumthermocellum DSM 1237. The coding sequences for bglA and bglB were located on non-homologous DNA fragments of 3.2- and 3.4-kb, respectively. Both genes direct in Escherichiacoli the synthesis of cytoplasmic β-glucosidases, which differ with respect to substrate specificity and temperature profile. The properties of the bglA-encoded β-glucosidase A closely resemble that of a β-glucosidase previously isolated from C.thermocellum cultures.

Original language	English
Pages (from-to)	73-78
Number of pages	6
Journal	Biotechnology Letters
Volume	10
Issue number	2
DOIs	https://doi.org/10.1007/BF01024629
State	Published - Feb 1988

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abstract = "Two β-glucosidase genes, designated bglA and bglB, were isolated from a gene bank of Clostridiumthermocellum DSM 1237. The coding sequences for bglA and bglB were located on non-homologous DNA fragments of 3.2- and 3.4-kb, respectively. Both genes direct in Escherichiacoli the synthesis of cytoplasmic β-glucosidases, which differ with respect to substrate specificity and temperature profile. The properties of the bglA-encoded β-glucosidase A closely resemble that of a β-glucosidase previously isolated from C.thermocellum cultures.",

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AB - Two β-glucosidase genes, designated bglA and bglB, were isolated from a gene bank of Clostridiumthermocellum DSM 1237. The coding sequences for bglA and bglB were located on non-homologous DNA fragments of 3.2- and 3.4-kb, respectively. Both genes direct in Escherichiacoli the synthesis of cytoplasmic β-glucosidases, which differ with respect to substrate specificity and temperature profile. The properties of the bglA-encoded β-glucosidase A closely resemble that of a β-glucosidase previously isolated from C.thermocellum cultures.

UR - https://www.scopus.com/pages/publications/0002611194

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JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 2

ER -

Characterization of two β-glucosidase genes from Clostridiumthermocellum

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