Characterization of the porcine AMPK alpha 2 catalytic subunit gene (PRKAA2): Genomic structure, polymorphism detection and association study

L. Lin, K. Flisikowski, H. Schwarzenbacher, M. Scharfe, S. Severitt, H. Blöcker, R. Fries

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

MP-activated protein kinase (AMPK), known as a key regulator of cellular energy homeostasis, plays an important role in regulation of glucose and lipid metabolism, and protein synthesis in mammals. The characterization of porcine PRKAA2 encoding the alpha 2 catalytic subunit of AMPK is reported in this study. PRKAA2 was assigned to porcine chromosome 6q by analysis of radiation hybrids (IMpRH panel), and its genomic structure was determined by BAC sequencing. PRKAA2 spans more than 62 kb and consists of nine exons and eight introns. A total of 25 polymorphisms were identified by re-sequencing approximately 7 kb, including all the exons, exon-intron boundaries and 5 and 3 gene flanking regions using twelve founder animals of a Mangalitsa Piétrain intercross. Neither of two single nucleotide polymorphisms (SNPs) found in the coding region caused an amino acid substitution. Two SNPs (NM-214266.1: c.236+142A<G and NM-214266.1: c.630C>T) in PRKAA2 were genotyped in the Mangalitsa Piétrain F2 cross (n = 589) and two commercial populations [Piétrain (n = 1173) and German Landrace (n = 536)] and evaluated for association with traits of interest (muscle development and fat deposition). Single SNP and haplotype analyses revealed weak associations between the PRKAA2 genotypes and loin muscle area in the investigated populations.

Original languageEnglish
Pages (from-to)203-207
Number of pages5
JournalAnimal Genetics
Volume41
Issue number2
DOIs
StatePublished - Apr 2010

Keywords

  • Association study
  • Genomic characterization
  • PRKAA2
  • Pigs

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