Characterization of dynamic processes using deuterium in uniformly 2H,13C,15N enriched peptides by MAS solid-state NMR

Maggy Hologne; Zhongjing Chen; Bernd Reif

doi:10.1016/j.jmr.2005.10.014

Characterization of dynamic processes using deuterium in uniformly ²H,¹³C,¹⁵N enriched peptides by MAS solid-state NMR

Maggy Hologne, Zhongjing Chen, Bernd Reif

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

We present in this paper ²H,¹³C MAS correlation experiments that are performed on a uniformly ²H,¹³C,¹⁵N labeled sample of Nac-Val, and on the uniformly ²H,¹⁵N labeled dipeptide Nac-Val-Leu-OH. The experiments involve the measurement of ²H T₁ relaxation times at two different magnetic fields, as well as the measurement of the ²H tensor parameters by evolution of the ²H chemical shift. The data are interpreted quantitatively to differentiate between different side chain motional models.

Original language	English
Pages (from-to)	20-28
Number of pages	9
Journal	Journal of Magnetic Resonance
Volume	179
Issue number	1
DOIs	https://doi.org/10.1016/j.jmr.2005.10.014
State	Published - Mar 2006
Externally published	Yes

Keywords

Deuteration
Dynamics
H
MAS solid-state NMR

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10.1016/j.jmr.2005.10.014

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title = "Characterization of dynamic processes using deuterium in uniformly 2H,13C,15N enriched peptides by MAS solid-state NMR",

abstract = "We present in this paper 2H,13C MAS correlation experiments that are performed on a uniformly 2H,13C,15N labeled sample of Nac-Val, and on the uniformly 2H,15N labeled dipeptide Nac-Val-Leu-OH. The experiments involve the measurement of 2H T1 relaxation times at two different magnetic fields, as well as the measurement of the 2H tensor parameters by evolution of the 2H chemical shift. The data are interpreted quantitatively to differentiate between different side chain motional models.",

keywords = "Deuteration, Dynamics, H, MAS solid-state NMR",

author = "Maggy Hologne and Zhongjing Chen and Bernd Reif",

note = "Funding Information: This research was supported by the DFG Grant Re1435.",

year = "2006",

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doi = "10.1016/j.jmr.2005.10.014",

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AU - Hologne, Maggy

AU - Chen, Zhongjing

AU - Reif, Bernd

N1 - Funding Information: This research was supported by the DFG Grant Re1435.

PY - 2006/3

Y1 - 2006/3

N2 - We present in this paper 2H,13C MAS correlation experiments that are performed on a uniformly 2H,13C,15N labeled sample of Nac-Val, and on the uniformly 2H,15N labeled dipeptide Nac-Val-Leu-OH. The experiments involve the measurement of 2H T1 relaxation times at two different magnetic fields, as well as the measurement of the 2H tensor parameters by evolution of the 2H chemical shift. The data are interpreted quantitatively to differentiate between different side chain motional models.

AB - We present in this paper 2H,13C MAS correlation experiments that are performed on a uniformly 2H,13C,15N labeled sample of Nac-Val, and on the uniformly 2H,15N labeled dipeptide Nac-Val-Leu-OH. The experiments involve the measurement of 2H T1 relaxation times at two different magnetic fields, as well as the measurement of the 2H tensor parameters by evolution of the 2H chemical shift. The data are interpreted quantitatively to differentiate between different side chain motional models.

KW - Deuteration

KW - Dynamics

KW - H

KW - MAS solid-state NMR

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SN - 1090-7807

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EP - 28

JO - Journal of Magnetic Resonance

JF - Journal of Magnetic Resonance

IS - 1

ER -

Characterization of dynamic processes using deuterium in uniformly ²H,¹³C,¹⁵N enriched peptides by MAS solid-state NMR

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Keywords

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