Characterization of cells and bacteria by photophoretic velocimetry

Clemens Helmbrecht, Reinhard Niessner, Christoph Haisch

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

The migration induced by intensive light is termed photophoresis. We could show that the evaluation of light-induced velocities of microparticles, bacteria and cells suspended in water is valuable for the prediction of their intrinsic properties. Two different laser setups were evaluated for photophoretic migration, a He-Ne laser (P = 45 mW, λ = 633 nm) and a diode-pumped cw-Nd:YAG (P = 1.1 W, λ = 532 nm). When analyzing the migration behavior of particles, we find significant differences depending on both, geometrical size and refractive index. We describe migration of PS particles of different size as well as with different refractive index but same diameter, SiO 2 and melamine resin. The potential for the separation of biological matter is shown as velocity distributions of heat killed bacteria of Escherichia coli, Salmonella enteritidis, and baker's yeast is reported.

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI
DOIs
StatePublished - 2008
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI - San Jose, CA, United States
Duration: 21 Jan 200823 Jan 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6859
ISSN (Print)1605-7422

Conference

ConferenceImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI
Country/TerritoryUnited States
CitySan Jose, CA
Period21/01/0823/01/08

Keywords

  • Photophoresis
  • microorganisms
  • microparticles
  • optical properties
  • radiation pressure

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