Characterization of Brown Adipose Tissue in a Diabetic Mouse Model with Spiral Volumetric Optoacoustic Tomography

Avihai Ron; Xosé Luís Deán-Ben; Josephine Reber; Vasilis Ntziachristos; Daniel Razansky

doi:10.1007/s11307-018-1291-y

Characterization of Brown Adipose Tissue in a Diabetic Mouse Model with Spiral Volumetric Optoacoustic Tomography

Avihai Ron, Xosé Luís Deán-Ben, Josephine Reber, Vasilis Ntziachristos, Daniel Razansky

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Purpose: Diabetes is associated with a deterioration of the microvasculature in brown adipose tissue (BAT) and with a decrease in its metabolic activity. Multispectral optoacoustic tomography has been recently proposed as a new tool capable of differentiating healthy and diabetic BAT by observing hemoglobin gradients and microvasculature density in cross-sectional (2D) views. We report on the use of spiral volumetric optoacoustic tomography (SVOT) for an improved characterization of BAT. Procedures: A streptozotocin-induced diabetes model and control mice were scanned with SVOT. Volumetric oxygen saturation (sO₂) as well as total blood volume (TBV) in the subcutaneous interscapular BAT (iBAT) was quantified. Segmentation further enabled separating feeding and draining vessels from the BAT anatomical structure. Results: Scanning revealed a 46 % decrease in TBV and a 25 % decrease in sO₂ in the diabetic iBAT with respect to the healthy control. Conclusions: These results suggest that SVOT may serve as an effective tool for studying the effects of diabetes on BAT. The volumetric optoacoustic imaging probe used for the SVOT scans can be operated in a handheld mode, thus potentially providing a clinical translation route for BAT-related studies with this imaging technology.

Original language	English
Pages (from-to)	620-625
Number of pages	6
Journal	Molecular Imaging and Biology
Volume	21
Issue number	4
DOIs	https://doi.org/10.1007/s11307-018-1291-y
State	Published - 15 Aug 2019

Keywords

Adipose tissue
Angiopathy
Brown fat
Hemoglobin
Metabolism
Optoacoustic
Oxygen saturation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11307-018-1291-y

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title = "Characterization of Brown Adipose Tissue in a Diabetic Mouse Model with Spiral Volumetric Optoacoustic Tomography",

abstract = "Purpose: Diabetes is associated with a deterioration of the microvasculature in brown adipose tissue (BAT) and with a decrease in its metabolic activity. Multispectral optoacoustic tomography has been recently proposed as a new tool capable of differentiating healthy and diabetic BAT by observing hemoglobin gradients and microvasculature density in cross-sectional (2D) views. We report on the use of spiral volumetric optoacoustic tomography (SVOT) for an improved characterization of BAT. Procedures: A streptozotocin-induced diabetes model and control mice were scanned with SVOT. Volumetric oxygen saturation (sO2) as well as total blood volume (TBV) in the subcutaneous interscapular BAT (iBAT) was quantified. Segmentation further enabled separating feeding and draining vessels from the BAT anatomical structure. Results: Scanning revealed a 46 % decrease in TBV and a 25 % decrease in sO2 in the diabetic iBAT with respect to the healthy control. Conclusions: These results suggest that SVOT may serve as an effective tool for studying the effects of diabetes on BAT. The volumetric optoacoustic imaging probe used for the SVOT scans can be operated in a handheld mode, thus potentially providing a clinical translation route for BAT-related studies with this imaging technology.",

keywords = "Adipose tissue, Angiopathy, Brown fat, Hemoglobin, Metabolism, Optoacoustic, Oxygen saturation",

author = "Avihai Ron and De{\'a}n-Ben, {Xos{\'e} Lu{\'i}s} and Josephine Reber and Vasilis Ntziachristos and Daniel Razansky",

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AU - Deán-Ben, Xosé Luís

AU - Reber, Josephine

AU - Ntziachristos, Vasilis

AU - Razansky, Daniel

PY - 2019/8/15

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N2 - Purpose: Diabetes is associated with a deterioration of the microvasculature in brown adipose tissue (BAT) and with a decrease in its metabolic activity. Multispectral optoacoustic tomography has been recently proposed as a new tool capable of differentiating healthy and diabetic BAT by observing hemoglobin gradients and microvasculature density in cross-sectional (2D) views. We report on the use of spiral volumetric optoacoustic tomography (SVOT) for an improved characterization of BAT. Procedures: A streptozotocin-induced diabetes model and control mice were scanned with SVOT. Volumetric oxygen saturation (sO2) as well as total blood volume (TBV) in the subcutaneous interscapular BAT (iBAT) was quantified. Segmentation further enabled separating feeding and draining vessels from the BAT anatomical structure. Results: Scanning revealed a 46 % decrease in TBV and a 25 % decrease in sO2 in the diabetic iBAT with respect to the healthy control. Conclusions: These results suggest that SVOT may serve as an effective tool for studying the effects of diabetes on BAT. The volumetric optoacoustic imaging probe used for the SVOT scans can be operated in a handheld mode, thus potentially providing a clinical translation route for BAT-related studies with this imaging technology.

AB - Purpose: Diabetes is associated with a deterioration of the microvasculature in brown adipose tissue (BAT) and with a decrease in its metabolic activity. Multispectral optoacoustic tomography has been recently proposed as a new tool capable of differentiating healthy and diabetic BAT by observing hemoglobin gradients and microvasculature density in cross-sectional (2D) views. We report on the use of spiral volumetric optoacoustic tomography (SVOT) for an improved characterization of BAT. Procedures: A streptozotocin-induced diabetes model and control mice were scanned with SVOT. Volumetric oxygen saturation (sO2) as well as total blood volume (TBV) in the subcutaneous interscapular BAT (iBAT) was quantified. Segmentation further enabled separating feeding and draining vessels from the BAT anatomical structure. Results: Scanning revealed a 46 % decrease in TBV and a 25 % decrease in sO2 in the diabetic iBAT with respect to the healthy control. Conclusions: These results suggest that SVOT may serve as an effective tool for studying the effects of diabetes on BAT. The volumetric optoacoustic imaging probe used for the SVOT scans can be operated in a handheld mode, thus potentially providing a clinical translation route for BAT-related studies with this imaging technology.

KW - Adipose tissue

KW - Angiopathy

KW - Brown fat

KW - Hemoglobin

KW - Metabolism

KW - Optoacoustic

KW - Oxygen saturation

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Characterization of Brown Adipose Tissue in a Diabetic Mouse Model with Spiral Volumetric Optoacoustic Tomography

Abstract

Keywords

UN SDGs

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