Characterization of Biomimetic Cofactors According to Stability, Redox Potentials, and Enzymatic Conversion by NADH Oxidase from Lactobacillus pentosus

Claudia Nowak, André Pick, Lénárd István Csepei, Volker Sieber

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Oxidoreductases are attractive biocatalysts that convert achiral substrates into products of higher value, but they are also for the most part dependent on nicotinamide cofactors. Recently, biomimetic nicotinamide derivatives have received attention as less costly alternatives to natural cofactors. However, recycling of biomimetics is still challenging because there are only limited opportunities. Here, we have characterized various biomimetic cofactors with regard to stability and redox potentials to find the best alternative to natural cofactors. Further, the cofactor spectrum of NADH oxidase from Lactobacillus pentosus (LpNox) could be expanded, and the enzymatic activity was also compared to activities with different small-molecule catalysts. As a result, we succeeded in identifying several strategies for regeneration of oxidized biomimetics.

Original languageEnglish
Pages (from-to)1944-1949
Number of pages6
JournalChemBioChem
Volume18
Issue number19
DOIs
StatePublished - 5 Oct 2017

Keywords

  • NADH
  • biomimetic cofactors
  • enzyme catalysis
  • oxidoreductases
  • redox chemistry

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