Abstract
We have generated a rat mAb, TR310, which recognizes a determinant encoded by the murine Vβ7 gene segment of the TCR. TR310 immunoprecipitates TCR from cell lysates, co-modulates with CD3, and can be used for immunofluorescence staining of T cells. By using this antibody, we found that the average percentage of Vβ7+ peripheral T cells in Mls-1b mice was 3.8%, but only 0.8% in Mls-1a mice. A similar difference was also observed in the mature TCR(hi) thymocyte subsets, suggesting that Vβ7+ T cells are deleted during intrathymic maturation in Mls-1a mice. TR310 should prove to be a valuable reagent in further studies of the TCR repertoire and the analysis of factors which alter it.
Original language | English |
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Pages (from-to) | 3473-3477 |
Number of pages | 5 |
Journal | Journal of Immunology |
Volume | 144 |
Issue number | 9 |
State | Published - 1990 |
Externally published | Yes |