TY - JOUR
T1 - Characterization and discrimination of pollen by Raman microscopy
AU - Ivleva, N. P.
AU - Niessner, R.
AU - Panne, U.
PY - 2005/1
Y1 - 2005/1
N2 - The chemical characterization and discrimination of allergy-relevant pollen (common ragweed (Ambrosia arfemisiifolia), white birch (Betula pendula), English oak (Quercus robur), and European linden (Tilia cordata)) has been studied by Raman microscopy. Spectra were obtained at different excitation wavelengths (514, 633, and 780 nm) and various methods were examined to minimize the strong fluorescence background. The use of a He-Ne laser (633 nm) for excitation yields high-quality single pollen Raman spectra, which contain multiple bands due to pollen components such as carotenoids, proteins, nucleic acids, carbohydrates, and lipids. Multivariate classification, i.e. principal component analysis (PCA) and hierarchical cluster analysis, demonstrated the validity of the approach for discrimination between different pollen species.
AB - The chemical characterization and discrimination of allergy-relevant pollen (common ragweed (Ambrosia arfemisiifolia), white birch (Betula pendula), English oak (Quercus robur), and European linden (Tilia cordata)) has been studied by Raman microscopy. Spectra were obtained at different excitation wavelengths (514, 633, and 780 nm) and various methods were examined to minimize the strong fluorescence background. The use of a He-Ne laser (633 nm) for excitation yields high-quality single pollen Raman spectra, which contain multiple bands due to pollen components such as carotenoids, proteins, nucleic acids, carbohydrates, and lipids. Multivariate classification, i.e. principal component analysis (PCA) and hierarchical cluster analysis, demonstrated the validity of the approach for discrimination between different pollen species.
KW - Multivariate classification
KW - Pollen
KW - Raman spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=13844254551&partnerID=8YFLogxK
U2 - 10.1007/s00216-004-2942-1
DO - 10.1007/s00216-004-2942-1
M3 - Article
C2 - 15605236
AN - SCOPUS:13844254551
SN - 1618-2642
VL - 381
SP - 261
EP - 267
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 1
ER -