TY - JOUR
T1 - Characterisation of the most odour-active compounds in a peel oil extract from Pontianak oranges (Citrus nobilis var. Lour. microcarpa Hassk.)
AU - Fischer, Anja
AU - Grab, Willi
AU - Schieberle, Peter
PY - 2008/7
Y1 - 2008/7
N2 - Isolation of the volatile fraction from the peel of Pontianak orange (Citrus nobilis var. Lour. microcarpa Hassk.) by a careful solvent extraction/vacuum distillation process followed by application of an aroma extract dilution analysis (AEDA) revealed 32 odour-active compounds in the flavour dilution (FD)-factor range of 4-2048, 26 of which could be identified. On the basis of high FD-factors, (R)/(S)-linalool, myrcene, (R)-limonene, and 1,8-cineole were characterised as the most potent odorants, followed by octanal, (E,E)-2,4-decadienal, nerol, (E)-2-dodecenal, geraniol, and (E,E)-2,4-nonadienal. In addition, one odorant resembling the characteristic sulphurous, resinous aroma of the Pontianak peel oil was detected with a quite high FD-factor of 128. By mass spectrometry followed by synthesis, 1-phenylethanethiol exhibiting an intense sulphurous, resinous smell at the very low odour threshold of 0.005 ng/L in air, was found to be responsible for the odour impression detected in the extract. 1-Phenylethanethiol occurring as a mixture of the (R)-(76%) and the (S)-enantiomer (24%) has previously not been reported as odorant in foods. Application of static headspace aroma dilution analysis (SHA) on Pontianak peel revealed the green, grassy smelling odour-active compounds hexanal and (Z)-3-hexenal as further important odorants in the headspace above the peels.
AB - Isolation of the volatile fraction from the peel of Pontianak orange (Citrus nobilis var. Lour. microcarpa Hassk.) by a careful solvent extraction/vacuum distillation process followed by application of an aroma extract dilution analysis (AEDA) revealed 32 odour-active compounds in the flavour dilution (FD)-factor range of 4-2048, 26 of which could be identified. On the basis of high FD-factors, (R)/(S)-linalool, myrcene, (R)-limonene, and 1,8-cineole were characterised as the most potent odorants, followed by octanal, (E,E)-2,4-decadienal, nerol, (E)-2-dodecenal, geraniol, and (E,E)-2,4-nonadienal. In addition, one odorant resembling the characteristic sulphurous, resinous aroma of the Pontianak peel oil was detected with a quite high FD-factor of 128. By mass spectrometry followed by synthesis, 1-phenylethanethiol exhibiting an intense sulphurous, resinous smell at the very low odour threshold of 0.005 ng/L in air, was found to be responsible for the odour impression detected in the extract. 1-Phenylethanethiol occurring as a mixture of the (R)-(76%) and the (S)-enantiomer (24%) has previously not been reported as odorant in foods. Application of static headspace aroma dilution analysis (SHA) on Pontianak peel revealed the green, grassy smelling odour-active compounds hexanal and (Z)-3-hexenal as further important odorants in the headspace above the peels.
KW - 1-Phenylethanethiol
KW - Aroma extract dilution analysis
KW - Mandarin
KW - Peel oil
KW - Pontianak orange
KW - Static headspace aroma dilution analysis
UR - http://www.scopus.com/inward/record.url?scp=46549084109&partnerID=8YFLogxK
U2 - 10.1007/s00217-007-0781-y
DO - 10.1007/s00217-007-0781-y
M3 - Article
AN - SCOPUS:46549084109
SN - 1438-2377
VL - 227
SP - 735
EP - 744
JO - European Food Research and Technology
JF - European Food Research and Technology
IS - 3
ER -