Characterisation of different polyclonal antisera to quantify Herbaspirillum spp. in elephant grass (Pennisetum purpureum Schun.)

V. M. Reis, Jr Dos Reis, J. F. Salles, M. Schloter

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Immunological techniques can be applied to detect micro-organisms in different ecosystems. To follow the fate of Herbaspirillum seropedicae strain Z67 and H. rubrisubalbicans strain M4 in the rhizosphere of elephant grass, Pennisetum purpureum Schun. var. Capim Cana D'Africa, polyclonal antisera were raised against both strains. Both antisera were purified with protein-A, followed by a primary characterisation for cross-reactivity. To reduce cross-reactivity for both sera, affinity purification was used for improvement. After that, both sera could be considered as species specific. The detection limit was 105 cells ml-1 for the anti H. rubrisubalbicans serum and 106 cells ml-1 for the anti H. seropedicae serum. It was shown that two months after inoculation, both strains could be detected in the rhizoplane of elephant grass in high densities. The estimated numbers by ELISA were 107 cells g-1 fresh weight.

Original languageEnglish
Pages (from-to)139-150
Number of pages12
JournalSymbiosis
Volume29
Issue number2
StatePublished - 2000
Externally publishedYes

Keywords

  • Diazotrophic bacteria
  • Herbaspirillum
  • Pennisetum purpureum
  • Polyclonal antisera

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