Characterisation of a piezotolerant mutant of Lactobacillus sanfranciscensis

Melanie Pavlovic, Sebastian Hörmann, Rudi F. Vogel, Matthias A. Ehrmann

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Incubation under sublethal high pressure (50 MPa) allowed the isolation of a piezotolerant mutant of Lactobacillus sanfranciscensis. Compared to the wild type this strain showed faster growth at 50 MPa and an altered temperature-dependent growth at ambient pressure. Additionally, an altered antibiotic resistance pattern was detected. To address the molecular basis of the mutation the genotypic characterisation was focused on alterations of ribosomal components. Northern analysis using ssrA (transfer mRNA) as probe revealed a constitutive overexpression in the mutant. A 2.2 fold induction after pressure shock and increased pressure sensitivity of a ssrA-insertional mutant of L. sanfranciscensis indicate the tmRNA as genetic determinant of a piezotolerance response in the wild type. Thus, we propose trans-translation and peptide tagging, processes that promote recycling of stalled ribosomes and prevent accumulation of abortively synthesised polypeptides to be involved in combating high-pressure damage and conferring moderate piezotolerance.

Original languageEnglish
Pages (from-to)791-797
Number of pages7
JournalZeitschrift fur Naturforschung - Section B Journal of Chemical Sciences
Volume63
Issue number6
DOIs
StatePublished - Jun 2008

Keywords

  • High pressure
  • Lactobacillus sanfranciscensis
  • Piezotolerance

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