Abstract
Treatment of male Wistar rats with 2-acetylaminofluorene (2-AAF) markedly altered the ploidy distribution of liver cells. Small diploid hepatocytes first appeared after 4-5 weeks feeding of a diet containing 0.02% 2-AAF; after 9 weeks 65-70% of the hepatocytes were diploid. Approximately two-thirds of this new liver cell population persisted after termination of the treatment. The hepatocytes from 2-AAF treated animals were separated according to size and ploidy by centrifugal elutriation and stained for γ-glutamyltrans-peptidase (γ-GTase). The percentage of γ-GTase-positive hepatocytes did not significantly differ between the various elutriated cell fractions. Thus γ-GTase-positive liver cells obtained by feeding of 2-AAF do not represent a distinct size class of hepatocytes. The significance of carcinogen-induced diploid hepatocytes in hepatocarcinogenesis is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 553-556 |
| Number of pages | 4 |
| Journal | Carcinogenesis |
| Volume | 10 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 1989 |
| Externally published | Yes |
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SDG 3 Good Health and Well-being
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