@article{4a662759e3c64a83bbe213908f67ca65,
title = "Centralized mouse repositories",
abstract = "Because the mouse is used so widely for biomedical research and the number of mouse models being generated is increasing rapidly, centralized repositories are essential if the valuable mouse strains and models that have been developed are to be securely preserved and fully exploited. Ensuring the ongoing availability of these mouse strains preserves the investment made in creating and characterizing them and creates a global resource of enormous value. The establishment of centralized mouse repositories around the world for distributing and archiving these resources has provided critical access to and preservation of these strains. This article describes the common and specialized activities provided by major mouse repositories around the world.",
author = "Donahue, {Leah Rae} and {De Angelis}, {Martin Hrabe} and Michael Hagn and Craig Franklin and {Kent Lloyd}, {K. C.} and Terry Magnuson and Colin McKerlie and Naomi Nakagata and Yuichi Obata and Stuart Read and Wolfgang Wurst and Andreas H{\"o}rlein and Davisson, {Muriel T.}",
note = "Funding Information: In 2007, the US National Institutes of Health (NIH) established a repository to archive and distribute mouse and ES cell lines produced by the KOMP. The KOMP is a trans-NIH initiative that created a comprehensive and public resource comprising mouse ES cell lines containing a null mutation in 8,500 of the ~22,000 genes in the mouse genome. The KOMP program targeted genes by homologous recombination of conditional-ready knockout-first, targeted-trap, and definitive null alleles in newly developed and improved C57BL/6 ES cells with robust germline transmission. Information and data generated by KOMP are collected and published online in a dedicated Data Coordination Center (DCC), which is freely accessible to the biomedical research community. KOMP production was funded by an NIH award to Velocigene, a division of Regeneron Pharmaceuticals, Inc. (Tarrytown, NY), and to a collaborative team from Children{\textquoteright}s Hospital Oakland Research Institute (CHORI) (Oakland, CA), the School of Veterinary Medicine, University of California, Davis (UC Davis), and the Wellcome Trust Sanger Institute (Hinxton, UK). A new NIH program, KOMP (Knockout Mouse Production and Phenotyping), which began in 2011, seeks to convert all of the KOMP-targeted ES cell lines to homozygous mutant mice for comprehensive, high-throughput, broad-based phenotyping. Data and information from the KOMP project will be made available online from a new international data center (iDCC). In addition, all targeting vectors, ES cells, mice, and germplasm are available for distribution from the KOMP Repository (Table ), a joint venture between UC Davis and CHORI. In addition to distribution of products, the KOMP Repository offers value-added services such as ES cell targeting, blastocyst microinjection of ES cells, cryopreservation and cryorecovery of germplasm, GQC testing of ES cells and mice, germline transmission testing, colony management, and a host of other production and phenotyping services. KOMP and KOMP are jointly funded by multiple NIH institutes, centers, and offices. 2 2 2 Funding Information: The nonprofit EMMA repository network collects, archives, and distributes biomedically important mutant mouse stocks. EMMA has a network of 14 partners across Europe. EMMA is supported by member institutional funds, national funding programs, and the European Commission{\textquoteright}s Framework Programmes. Strain information for all EMMA strains, online submission and order forms, and links to the home page of individual EMMA members may be found at the EMMA web site (Table ). EMMA offers as a special service the generation of germ-free (axenic) mice ( http://www.emmanet.org/axenic/intro.php ). EMMA also does research to establish the optimum conditions for transportation of unfrozen preimplantation embryos across international boundaries. Furthermore, EMMA offers extensive training opportunities with currently five annual training courses. A comprehensive collection of up-to-date protocols taught in the cryopreservation courses is displayed on the EMMA website. The EMMA archive currently comprises 3,000 mouse mutant lines, among them the highly demanded EUCOMM mouse resource and the Wellcome Trust KO Mouse Resource. The EMMA mouse mutant collection can be searched by strains, genes, phenotypes, and human diseases (Table ). ",
year = "2012",
month = oct,
doi = "10.1007/s00335-012-9420-4",
language = "English",
volume = "23",
pages = "559--571",
journal = "Mammalian Genome",
issn = "0938-8990",
publisher = "Springer New York",
number = "9-10",
}