Cellular solid-phase binding assay and mass spectrometry for screening of α4β7 integrin antagonists

Dirk Gottschling; Jürgen Boer; Anja Schuster; Bernhard Holzmann; Horst Kessler

doi:10.1016/S0960-894X(01)00611-4

Cellular solid-phase binding assay and mass spectrometry for screening of α4β7 integrin antagonists

Dirk Gottschling, Jürgen Boer, Anja Schuster, Bernhard Holzmann, Horst Kessler

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A qualitative cellular solid-phase binding assay for screening α4β7 integrin antagonists attached via photolinker to TentaGel® Macrobeads has been developed. An activation of the integrins with Mn²⁺ was necessary to achieve binding to the bead bound antagonists. The identification of the resin bound compounds was done by mass spectrometry.

Original language	English
Pages (from-to)	2997-3000
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	11
Issue number	23
DOIs	https://doi.org/10.1016/S0960-894X(01)00611-4
State	Published - 3 Dec 2001

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10.1016/S0960-894X(01)00611-4

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title = "Cellular solid-phase binding assay and mass spectrometry for screening of α4β7 integrin antagonists",

abstract = "A qualitative cellular solid-phase binding assay for screening α4β7 integrin antagonists attached via photolinker to TentaGel{\textregistered} Macrobeads has been developed. An activation of the integrins with Mn2+ was necessary to achieve binding to the bead bound antagonists. The identification of the resin bound compounds was done by mass spectrometry.",

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AU - Gottschling, Dirk

AU - Boer, Jürgen

AU - Schuster, Anja

AU - Holzmann, Bernhard

AU - Kessler, Horst

PY - 2001/12/3

Y1 - 2001/12/3

N2 - A qualitative cellular solid-phase binding assay for screening α4β7 integrin antagonists attached via photolinker to TentaGel® Macrobeads has been developed. An activation of the integrins with Mn2+ was necessary to achieve binding to the bead bound antagonists. The identification of the resin bound compounds was done by mass spectrometry.

AB - A qualitative cellular solid-phase binding assay for screening α4β7 integrin antagonists attached via photolinker to TentaGel® Macrobeads has been developed. An activation of the integrins with Mn2+ was necessary to achieve binding to the bead bound antagonists. The identification of the resin bound compounds was done by mass spectrometry.

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U2 - 10.1016/S0960-894X(01)00611-4

DO - 10.1016/S0960-894X(01)00611-4

M3 - Article

C2 - 11714596

AN - SCOPUS:0035803052

SN - 0960-894X

VL - 11

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EP - 3000

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 23

ER -

Cellular solid-phase binding assay and mass spectrometry for screening of α4β7 integrin antagonists

Abstract

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