Cellular localization of cytochrome c553 in the N2-fixing cyanobacterium Anabaena variabilis

Aurelio Serrano, Patricia Giménez, Siegfried Scherer, Peter Böger

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The "in situ" location of the electron carrier protein cytochrome C553 (cyt c553) has been investigated in both vegetative cells and heterocysts of the cyanobacterium Anabaena variabilis ATCC 29413 using the antibody-gold technique, carried out as a post-ernbedding immunoelectron microscopy procedure. When using a rabbit polyclonal anti-cyt c553 specific antiserum an intense labelling, associated mainly with the cell periphery (cytoplasmic membrane and periplasmic area), was seen in both heterocysts and vegetative cells. The selective release of most of the cellular cyt c553 during a Tris-EDTA treatment confirms a periplasmic localization of this protein in A. variabilis. The results indicate that most of cyt c553 is located in the periplasmic space. The roles ascribed to this protein in both respiration and photosynthesis in cyanobacteria are discussed.

Original languageEnglish
Pages (from-to)614-618
Number of pages5
JournalArchives of Microbiology
Volume154
Issue number6
DOIs
StatePublished - Nov 1990
Externally publishedYes

Keywords

  • Anabaena
  • Blue-green algae
  • Cytochrome c
  • Electron transport chain
  • Immunogold labelling
  • Photosynthesis
  • Protein localization
  • Respiration

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