Abstract
The "in situ" location of the electron carrier protein cytochrome C553 (cyt c553) has been investigated in both vegetative cells and heterocysts of the cyanobacterium Anabaena variabilis ATCC 29413 using the antibody-gold technique, carried out as a post-ernbedding immunoelectron microscopy procedure. When using a rabbit polyclonal anti-cyt c553 specific antiserum an intense labelling, associated mainly with the cell periphery (cytoplasmic membrane and periplasmic area), was seen in both heterocysts and vegetative cells. The selective release of most of the cellular cyt c553 during a Tris-EDTA treatment confirms a periplasmic localization of this protein in A. variabilis. The results indicate that most of cyt c553 is located in the periplasmic space. The roles ascribed to this protein in both respiration and photosynthesis in cyanobacteria are discussed.
Original language | English |
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Pages (from-to) | 614-618 |
Number of pages | 5 |
Journal | Archives of Microbiology |
Volume | 154 |
Issue number | 6 |
DOIs | |
State | Published - Nov 1990 |
Externally published | Yes |
Keywords
- Anabaena
- Blue-green algae
- Cytochrome c
- Electron transport chain
- Immunogold labelling
- Photosynthesis
- Protein localization
- Respiration