Cell mediated immunity in canine marrow graft recipients given cyclophosphamide

J. Abb, H. J. Kolb, H. Grosse-Wilde, I. Reider, S. Thierfelder

Research output: Contribution to journalArticlepeer-review

Abstract

The cellular immune status of 48 canine chimeras was studied between 15 days and 3 yr after a lethal dose of cyclophosphamide (CY) and an allogeneic marrow graft. Marrow graft recipients were given no further immunosuppressive treatment. Recovery of total white blood cell counts was complete by day 20 after grafting. Peripheral blood lymphocyte counts did not reach the normal range until day 100. Short term chimeras (less than 100 days postgrafting) showed marked impairment of cell mediated immunity as indicated by the inability to respond adequately to nonspecific mitogenic (PHA, ConA, PWM) and specific antigenic (allogeneic lymphocytes, PPD) stimulation in vitro. Skin test reactivity to PPD after immunization with BCG was also significantly depressed. Long term chimeras (more than 100 days postgrafting) had normal in vitro response to mitogens; lymphocyte blastogenesis to specific antigens however remained decreased for prolonged periods postgrafting. The survival time of first set allogeneic skin grafts showed no significant difference between chimeras and normal control dogs. Lymphocyte transformation in response to stimulation with specific antigens may prove a useful parameter for the in vitro evaluation of defects in cellular immune reactivity following allogeneic marrow transplantation.

Original languageEnglish
Pages (from-to)58-66
Number of pages9
JournalExperimental Hematology
Volume6
Issue number1
StatePublished - 1978
Externally publishedYes

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