Cell line dependent RNA expression profiles of prion-infected mouse neuronal cells

Alex D. Greenwood; Marion Horsch; Anna Stengel; Ina Vorberg; Gloria Lutzny; Elke Maas; Sandra Schädler; Volker Erfle; Johannes Beckers; Hermann Schätzl; Christine Leib-Mösch

doi:10.1016/j.jmb.2005.03.076

Cell line dependent RNA expression profiles of prion-infected mouse neuronal cells

Alex D. Greenwood, Marion Horsch, Anna Stengel, Ina Vorberg, Gloria Lutzny, Elke Maas, Sandra Schädler, Volker Erfle, Johannes Beckers, Hermann Schätzl, Christine Leib-Mösch

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

The overall impact of prion disease on gene expression is not well characterized. We have carried out a large-scale expression analysis of specific cell types commonly employed in studies of prion disease. Neuroblastoma cells (N2a) and hypothalamic neuronal cells (GT1) can be persistently infected with mouse-adapted scrapie prions, the latter demonstrating cytopathologic effects associated with prion neuropathology. Exploiting a mouse DNA microarray containing ∼21,000 spotted cDNAs, we have identified several hundred differentially expressed sequences in the two cell lines when infected with prion strain RML. ScN2a and ScGT1 cells demonstrate unique changes in RNA profiles and both differ from the reported changes in human microglia and prion-infected brain studies albeit with some overlap. In addition, several of the identified changes are shared in common with other neurodegenerative diseases such as Alzheimer's disease. The results illustrate that prion infection differs in effect depending on cell type, which could be exploited for diagnostic or therapeutic intervention.

Original language	English
Pages (from-to)	487-500
Number of pages	14
Journal	Journal of Molecular Biology
Volume	349
Issue number	3
DOIs	https://doi.org/10.1016/j.jmb.2005.03.076
State	Published - 10 Jun 2005
Externally published	Yes

Keywords

DNA microarray
Gene expression
Hypothalamus
Neuroblastoma
Prion

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jmb.2005.03.076

Cite this

@article{740758c8b87642f3a4ef274e0d55f4c5,

title = "Cell line dependent RNA expression profiles of prion-infected mouse neuronal cells",

abstract = "The overall impact of prion disease on gene expression is not well characterized. We have carried out a large-scale expression analysis of specific cell types commonly employed in studies of prion disease. Neuroblastoma cells (N2a) and hypothalamic neuronal cells (GT1) can be persistently infected with mouse-adapted scrapie prions, the latter demonstrating cytopathologic effects associated with prion neuropathology. Exploiting a mouse DNA microarray containing ∼21,000 spotted cDNAs, we have identified several hundred differentially expressed sequences in the two cell lines when infected with prion strain RML. ScN2a and ScGT1 cells demonstrate unique changes in RNA profiles and both differ from the reported changes in human microglia and prion-infected brain studies albeit with some overlap. In addition, several of the identified changes are shared in common with other neurodegenerative diseases such as Alzheimer's disease. The results illustrate that prion infection differs in effect depending on cell type, which could be exploited for diagnostic or therapeutic intervention.",

keywords = "DNA microarray, Gene expression, Hypothalamus, Neuroblastoma, Prion",

author = "Greenwood, {Alex D.} and Marion Horsch and Anna Stengel and Ina Vorberg and Gloria Lutzny and Elke Maas and Sandra Sch{\"a}dler and Volker Erfle and Johannes Beckers and Hermann Sch{\"a}tzl and Christine Leib-M{\"o}sch",

note = "Funding Information: The authors thank C. Englbrecht, A. Facius and P. Pagel for assistance with the microarray data analysis. Financial support for A.G., I.V., E.M., V.E., H.S., and C.L.-M. was provided by the Bayerischer Forschungsverbund Prionen (FORPRION) grant numbers TUM 7 and LMU 5. I.V., E.M., and H.S. were also supported by grants SFB-596 and DFG (Scha594/3-4) and by the EU NoE Neuroprion. M.H., S.S. and J.B. were supported by funds from the German National Genome Research Network (NGFN).",

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doi = "10.1016/j.jmb.2005.03.076",

language = "English",

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T1 - Cell line dependent RNA expression profiles of prion-infected mouse neuronal cells

AU - Greenwood, Alex D.

AU - Horsch, Marion

AU - Stengel, Anna

AU - Vorberg, Ina

AU - Lutzny, Gloria

AU - Maas, Elke

AU - Schädler, Sandra

AU - Erfle, Volker

AU - Beckers, Johannes

AU - Schätzl, Hermann

AU - Leib-Mösch, Christine

N1 - Funding Information: The authors thank C. Englbrecht, A. Facius and P. Pagel for assistance with the microarray data analysis. Financial support for A.G., I.V., E.M., V.E., H.S., and C.L.-M. was provided by the Bayerischer Forschungsverbund Prionen (FORPRION) grant numbers TUM 7 and LMU 5. I.V., E.M., and H.S. were also supported by grants SFB-596 and DFG (Scha594/3-4) and by the EU NoE Neuroprion. M.H., S.S. and J.B. were supported by funds from the German National Genome Research Network (NGFN).

PY - 2005/6/10

Y1 - 2005/6/10

N2 - The overall impact of prion disease on gene expression is not well characterized. We have carried out a large-scale expression analysis of specific cell types commonly employed in studies of prion disease. Neuroblastoma cells (N2a) and hypothalamic neuronal cells (GT1) can be persistently infected with mouse-adapted scrapie prions, the latter demonstrating cytopathologic effects associated with prion neuropathology. Exploiting a mouse DNA microarray containing ∼21,000 spotted cDNAs, we have identified several hundred differentially expressed sequences in the two cell lines when infected with prion strain RML. ScN2a and ScGT1 cells demonstrate unique changes in RNA profiles and both differ from the reported changes in human microglia and prion-infected brain studies albeit with some overlap. In addition, several of the identified changes are shared in common with other neurodegenerative diseases such as Alzheimer's disease. The results illustrate that prion infection differs in effect depending on cell type, which could be exploited for diagnostic or therapeutic intervention.

AB - The overall impact of prion disease on gene expression is not well characterized. We have carried out a large-scale expression analysis of specific cell types commonly employed in studies of prion disease. Neuroblastoma cells (N2a) and hypothalamic neuronal cells (GT1) can be persistently infected with mouse-adapted scrapie prions, the latter demonstrating cytopathologic effects associated with prion neuropathology. Exploiting a mouse DNA microarray containing ∼21,000 spotted cDNAs, we have identified several hundred differentially expressed sequences in the two cell lines when infected with prion strain RML. ScN2a and ScGT1 cells demonstrate unique changes in RNA profiles and both differ from the reported changes in human microglia and prion-infected brain studies albeit with some overlap. In addition, several of the identified changes are shared in common with other neurodegenerative diseases such as Alzheimer's disease. The results illustrate that prion infection differs in effect depending on cell type, which could be exploited for diagnostic or therapeutic intervention.

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KW - Gene expression

KW - Hypothalamus

KW - Neuroblastoma

KW - Prion

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EP - 500

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JF - Journal of Molecular Biology

IS - 3

ER -

Cell line dependent RNA expression profiles of prion-infected mouse neuronal cells

Abstract

Keywords

UN SDGs

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