Cell-free synthesis of glyoxysomal malate dehydrogenase

R. A. Walk, B. Hock

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


Polyadenylated mRNA was isolated from germinating watermelon cotyledons and translated in a wheat germ protein synthesizing system. The synthesis of glyoxysomal malate dehydrogenase was detected by direct immunoprecipitation and electrophoretic analysis of the precipitate. In addition to a small amount of the authentic isoenzyme (subunit molecular weight = 33 000), the major part of the incorporated [35S] methionine was observed in a polypeptide with a molecular weight of 38 000. The possible role of the larger molecule as a precursor of glyoxysomal malate dehydrogenase is discussed.

Original languageEnglish
Pages (from-to)636-643
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - 30 Mar 1978
Externally publishedYes


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