Cell-free enzyme cascades — application and transition from development to industrial implementation

In the vision to realize a circular economy aiming for net carbon neutrality or even negativity, cell-free bioconversion of sustainable and renewable resources emerged as a promising strategy. The potential of in vitro systems is enormous, delivering technological, ecological, and ethical added values. Innovative concepts arose in cell-free enzymatic conversions to reduce process waste production and preserve fossil resources, as well as to redirect and assimilate released industrial pollutions back into the production cycle again. However, the great challenge in the near future will be the jump from a concept to an industrial application. The transition process in industrial implementation also requires economic aspects such as productivity, scalability, and cost-effectiveness. Here, we briefly review the latest proof-of-concept cascades using carbon dioxide and other C1 or lignocellulose-derived chemicals as blueprints to efficiently recycle greenhouse gases, as well as cutting-edge technologies to maturate these concepts to industrial pilot plants.