Cell cycle-regulated PLEIADE/AtMAP65-3 links membrane and microtubule dynamics during plant cytokinesis

Alexander Steiner, Katarzyna Rybak, Melina Altmann, Heather E. McFarlane, Susan Klaeger, Ngoc Nguyen, Eva Facher, Alexander Ivakov, Gerhard Wanner, Bernhard Kuster, Staffan Persson, Pascal Braun, Marie Theres Hauser, Farhah F. Assaad

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Cytokinesis, the partitioning of the cytoplasm following nuclear division, requires extensive coordination between cell cycle cues, membrane trafficking and microtubule dynamics. Plant cytokinesis occurs within a transient membrane compartment known as the cell plate, to which vesicles are delivered by a plant-specific microtubule array, the phragmoplast. While membrane proteins required for cytokinesis are known, how these are coordinated with microtubule dynamics and regulated by cell cycle cues remains unclear. Here, we document physical and genetic interactions between Transport Protein Particle II (TRAPPII) tethering factors and microtubule-associated proteins of the PLEIADE/AtMAP65 family. These interactions do not specifically affect the recruitment of either TRAPPII or MAP65 proteins to the cell plate or midzone. Rather, and based on single versus double mutant phenotypes, it appears that they are required to coordinate cytokinesis with the nuclear division cycle. As MAP65 family members are known to be targets of cell cycle-regulated kinases, our results provide a conceptual framework for how membrane and microtubule dynamics may be coordinated with each other and with the nuclear cycle during plant cytokinesis.

Original languageEnglish
Pages (from-to)531-541
Number of pages11
JournalPlant Journal
Volume88
Issue number4
DOIs
StatePublished - 1 Nov 2016

Keywords

  • Arabidopsis thaliana
  • AtTRS120
  • CLUB/TRS130
  • MAP65-3/PLEAIDE
  • TRAPPII
  • cell plate
  • cytokinesis
  • phragmoplast microtubules

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