CD4+ T Cell Epitope Identification from Complex Parasite Antigen Mixtures

Miguel Álvaro-Benito; Friederike Ebner; Miriam Bertazzon; Eliot Morrison

doi:10.1007/978-1-0716-3239-0_6

CD4+ T Cell Epitope Identification from Complex Parasite Antigen Mixtures

Miguel Álvaro-Benito
, Friederike Ebner
, Miriam Bertazzon
, Eliot Morrison

Assistant Professorship of Infection Pathogenesis

Free University of Berlin

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Antigen complexity represents a major challenge for scoring CD4+ T cell immunogenicity, a key hallmark of immunity and with great potential to improve vaccine development. In this chapter, we provide a comprehensive picture of a pipeline that can be applied to virtually any complex antigen to overcome different limitations. Antigens are characterized by Mass Spectrometry to determine the available protein sources and their abundances. A reconstituted in vitro antigen processing system is applied along with bioinformatics tools to prioritize the list of candidates. Finally, the immunogenicity of candidate peptides is validated ex vivo using PBMCs from HLA-typed individuals. This protocol compiles the essential information for executing the whole pipeline while focusing on the candidate epitope prioritizing scheme.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	89-109
Number of pages	21
DOIs	https://doi.org/10.1007/978-1-0716-3239-0_6
State	Published - 2023

Publication series

Name	Methods in Molecular Biology
Volume	2673
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Antigen presentation
Ascaris spp. parasites
CD4+ T cell reactivity
Complex antigen mixtures
Excretory-secretory products (ES)
MHC II
Reconstitution in vitro antigen processing

Access to Document

10.1007/978-1-0716-3239-0_6

Cite this

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abstract = "Antigen complexity represents a major challenge for scoring CD4+ T cell immunogenicity, a key hallmark of immunity and with great potential to improve vaccine development. In this chapter, we provide a comprehensive picture of a pipeline that can be applied to virtually any complex antigen to overcome different limitations. Antigens are characterized by Mass Spectrometry to determine the available protein sources and their abundances. A reconstituted in vitro antigen processing system is applied along with bioinformatics tools to prioritize the list of candidates. Finally, the immunogenicity of candidate peptides is validated ex vivo using PBMCs from HLA-typed individuals. This protocol compiles the essential information for executing the whole pipeline while focusing on the candidate epitope prioritizing scheme.",

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AU - Bertazzon, Miriam

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KW - Antigen presentation

KW - Ascaris spp. parasites

KW - CD4+ T cell reactivity

KW - Complex antigen mixtures

KW - Excretory-secretory products (ES)

KW - MHC II

KW - Reconstitution in vitro antigen processing

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M3 - Chapter

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AN - SCOPUS:85160776111

T3 - Methods in Molecular Biology

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

CD4+ T Cell Epitope Identification from Complex Parasite Antigen Mixtures

Abstract

Publication series

UN SDGs

Keywords

Access to Document

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