Capillary electrochromatography and on-line concentration.

Guichen Ping, Philippe Schmitt-Kopplin, Yukui Zhang, Yoshinobu Baba

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Capillary electrochromatography (CEC) is a micro-separation technique that combines the advantages of capillary zone electrophoresis with those of high-performance liquid chromatography. Accordingly, it has attracted extensive attention over the last decade. Among the stationary phases for CEC, monolithic stationary phase has been regarded as the most suitable stationary phase for CEC because of its simple preparation, the elimination of frits, and its excellent performance. In this chapter, procedures for preparing CEC monolithic columns with an improved configuration, in which there are stationary phases at both sides of detection window and no stationary phase at detection window, are presented. The separation of acidic and basic compounds on such monolithic columns is used as an example to demonstrate CEC separation protocol. Additionally, an on-line concentration technique in CEC is presented. As a result of the coexistence of stationary phase and electric field in a CEC column, it is possible to employ chromatographic zone sharpening and field-amplified sample stacking effects simultaneously to improve CEC detection sensitivity.

Original languageEnglish
Pages (from-to)751-769
Number of pages19
JournalMethods in molecular biology (Clifton, N.J.)
Volume384
DOIs
StatePublished - 2008
Externally publishedYes

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