Canine-DCs using different serum-free methods as an approach to provide an animal-model for immunotherapeutic strategies

Dagmar Bund, Raymund Buhmann, Füsun Gökmen, Andreas Kremser, Julia Dreyssig, Hans Jochem Kolb, Helga Maria Schmetzer

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Animal-models are the basis of DC-based human immunotherapies. We describe the standardization of a canine-DC-generation protocol using different cytokines and characterize the quality and functional repertoire of the obtained canine-DCs. DCs were generated from healthy dog-PBMCs under serum-free and serum-containing conditions. DC-quality and -quantity was determined by FACS studying the expression-profiles of DC-/costimulatory- and maturation-antigens before/after culture with canine and human monoclonal-antibodies (cmabs/hmabs). Individual DCAgs-(DC-antigens)-expression-profiles were found before and after culture depending on the agents' mode-of-action. With at least one of three serum-free methods (. Ca-Ionophore, Picibanil, Cytokines) sufficient DC-amounts were generated. So, canine-DCs can be regularly generated under serum-free conditions and hmabs additionally to cmabs qualify for staining/quantification of canine-cells/DCs. The canine-DCs were functional, shown by T-cell-activation, -proliferation and antigen-specific CTL-responses. In summary, successful, quantitative DC-generation is possible with serum-free methods. DC-based T-cell-vaccination-strategies evaluated for e.g. AML-patients can be tested in the dog and estimated in clinical studies for DC-vaccination-strategies.

Original languageEnglish
Pages (from-to)88-98
Number of pages11
JournalCellular Immunology
Volume263
Issue number1
DOIs
StatePublished - 2010
Externally publishedYes

Keywords

  • Canine model
  • Dendritic cells
  • Immunotherapy
  • Serum-free culture

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