Calcium Sensor for Photoacoustic Imaging

Sheryl Roberts; Markus Seeger; Yuanyuan Jiang; Anurag Mishra; Felix Sigmund; Anja Stelzl; Antonella Lauri; Panagiotis Symvoulidis; Hannes Rolbieski; Matthias Preller; X. Luís Deán-Ben; Daniel Razansky; Tanja Orschmann; Sabrina C. Desbordes; Paul Vetschera; Thorsten Bach; Vasilis Ntziachristos; Gil G. Westmeyer

doi:10.1021/jacs.7b03064

Calcium Sensor for Photoacoustic Imaging

Sheryl Roberts, Markus Seeger, Yuanyuan Jiang, Anurag Mishra, Felix Sigmund, Anja Stelzl, Antonella Lauri, Panagiotis Symvoulidis, Hannes Rolbieski, Matthias Preller, X. Luís Deán-Ben, Daniel Razansky, Tanja Orschmann, Sabrina C. Desbordes, Paul Vetschera, Thorsten Bach, Vasilis Ntziachristos, Gil G. Westmeyer

Research output: Contribution to journal › Article › peer-review

111 Scopus citations

Abstract

We introduce a selective and cell-permeable calcium sensor for photoacoustics (CaSPA), a versatile imaging technique that allows for fast volumetric mapping of photoabsorbing molecules with deep tissue penetration. To optimize for Ca²⁺-dependent photoacoustic signal changes, we synthesized a selective metallochromic sensor with high extinction coefficient, low quantum yield, and high photobleaching resistance. Micromolar concentrations of Ca²⁺ lead to a robust blueshift of the absorbance of CaSPA, which translated into an accompanying decrease of the peak photoacoustic signal. The acetoxymethyl esterified sensor variant was readily taken up by cells without toxic effects and thus allowed us for the first time to perform live imaging of Ca²⁺ fluxes in genetically unmodified cells and heart organoids as well as in zebrafish larval brain via combined fluorescence and photoacoustic imaging.

Original language	English
Pages (from-to)	2718-2721
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	140
Issue number	8
DOIs	https://doi.org/10.1021/jacs.7b03064
State	Published - 28 Feb 2018

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Roberts, S., Seeger, M., Jiang, Y., Mishra, A., Sigmund, F., Stelzl, A., Lauri, A., Symvoulidis, P., Rolbieski, H., Preller, M., Deán-Ben, X. L., Razansky, D., Orschmann, T., Desbordes, S. C., Vetschera, P., Bach, T., Ntziachristos, V., & Westmeyer, G. G. (2018). Calcium Sensor for Photoacoustic Imaging. Journal of the American Chemical Society, 140(8), 2718-2721. https://doi.org/10.1021/jacs.7b03064

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title = "Calcium Sensor for Photoacoustic Imaging",

abstract = "We introduce a selective and cell-permeable calcium sensor for photoacoustics (CaSPA), a versatile imaging technique that allows for fast volumetric mapping of photoabsorbing molecules with deep tissue penetration. To optimize for Ca2+-dependent photoacoustic signal changes, we synthesized a selective metallochromic sensor with high extinction coefficient, low quantum yield, and high photobleaching resistance. Micromolar concentrations of Ca2+ lead to a robust blueshift of the absorbance of CaSPA, which translated into an accompanying decrease of the peak photoacoustic signal. The acetoxymethyl esterified sensor variant was readily taken up by cells without toxic effects and thus allowed us for the first time to perform live imaging of Ca2+ fluxes in genetically unmodified cells and heart organoids as well as in zebrafish larval brain via combined fluorescence and photoacoustic imaging.",

author = "Sheryl Roberts and Markus Seeger and Yuanyuan Jiang and Anurag Mishra and Felix Sigmund and Anja Stelzl and Antonella Lauri and Panagiotis Symvoulidis and Hannes Rolbieski and Matthias Preller and De{\'a}n-Ben, {X. Lu{\'i}s} and Daniel Razansky and Tanja Orschmann and Desbordes, {Sabrina C.} and Paul Vetschera and Thorsten Bach and Vasilis Ntziachristos and Westmeyer, {Gil G.}",

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doi = "10.1021/jacs.7b03064",

language = "English",

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Roberts, S, Seeger, M, Jiang, Y, Mishra, A, Sigmund, F, Stelzl, A, Lauri, A, Symvoulidis, P, Rolbieski, H, Preller, M, Deán-Ben, XL, Razansky, D, Orschmann, T, Desbordes, SC, Vetschera, P, Bach, T , Ntziachristos, V & Westmeyer, GG 2018, 'Calcium Sensor for Photoacoustic Imaging', Journal of the American Chemical Society, vol. 140, no. 8, pp. 2718-2721. https://doi.org/10.1021/jacs.7b03064

TY - JOUR

T1 - Calcium Sensor for Photoacoustic Imaging

AU - Roberts, Sheryl

AU - Seeger, Markus

AU - Jiang, Yuanyuan

AU - Mishra, Anurag

AU - Sigmund, Felix

AU - Stelzl, Anja

AU - Lauri, Antonella

AU - Symvoulidis, Panagiotis

AU - Rolbieski, Hannes

AU - Preller, Matthias

AU - Deán-Ben, X. Luís

AU - Razansky, Daniel

AU - Orschmann, Tanja

AU - Desbordes, Sabrina C.

AU - Vetschera, Paul

AU - Bach, Thorsten

AU - Ntziachristos, Vasilis

AU - Westmeyer, Gil G.

PY - 2018/2/28

Y1 - 2018/2/28

N2 - We introduce a selective and cell-permeable calcium sensor for photoacoustics (CaSPA), a versatile imaging technique that allows for fast volumetric mapping of photoabsorbing molecules with deep tissue penetration. To optimize for Ca2+-dependent photoacoustic signal changes, we synthesized a selective metallochromic sensor with high extinction coefficient, low quantum yield, and high photobleaching resistance. Micromolar concentrations of Ca2+ lead to a robust blueshift of the absorbance of CaSPA, which translated into an accompanying decrease of the peak photoacoustic signal. The acetoxymethyl esterified sensor variant was readily taken up by cells without toxic effects and thus allowed us for the first time to perform live imaging of Ca2+ fluxes in genetically unmodified cells and heart organoids as well as in zebrafish larval brain via combined fluorescence and photoacoustic imaging.

AB - We introduce a selective and cell-permeable calcium sensor for photoacoustics (CaSPA), a versatile imaging technique that allows for fast volumetric mapping of photoabsorbing molecules with deep tissue penetration. To optimize for Ca2+-dependent photoacoustic signal changes, we synthesized a selective metallochromic sensor with high extinction coefficient, low quantum yield, and high photobleaching resistance. Micromolar concentrations of Ca2+ lead to a robust blueshift of the absorbance of CaSPA, which translated into an accompanying decrease of the peak photoacoustic signal. The acetoxymethyl esterified sensor variant was readily taken up by cells without toxic effects and thus allowed us for the first time to perform live imaging of Ca2+ fluxes in genetically unmodified cells and heart organoids as well as in zebrafish larval brain via combined fluorescence and photoacoustic imaging.

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VL - 140

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JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

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ER -

Calcium Sensor for Photoacoustic Imaging

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