Building ProteomeTools based on a complete synthetic human proteome

Daniel P. Zolg; Mathias Wilhelm; Karsten Schnatbaum; Johannes Zerweck; Tobias Knaute; Bernard Delanghe; Derek J. Bailey; Siegfried Gessulat; Hans Christian Ehrlich; Maximilian Weininger; Peng Yu; Judith Schlegl; Karl Kramer; Tobias Schmidt; Ulrike Kusebauch; Eric W. Deutsch; Ruedi Aebersold; Robert L. Moritz; Holger Wenschuh; Thomas Moehring; Stephan Aiche; Andreas Huhmer; Ulf Reimer; Bernhard Kuster

doi:10.1038/nmeth.4153

Building ProteomeTools based on a complete synthetic human proteome

Daniel P. Zolg, Mathias Wilhelm, Karsten Schnatbaum, Johannes Zerweck, Tobias Knaute, Bernard Delanghe, Derek J. Bailey, Siegfried Gessulat, Hans Christian Ehrlich, Maximilian Weininger, Peng Yu, Judith Schlegl, Karl Kramer, Tobias Schmidt, Ulrike Kusebauch, Eric W. Deutsch, Ruedi Aebersold, Robert L. Moritz, Holger Wenschuh, Thomas MoehringStephan Aiche, Andreas Huhmer, Ulf Reimer, Bernhard Kuster

Research output: Contribution to journal › Article › peer-review

162 Scopus citations

Abstract

We describe ProteomeTools, a project building molecular and digital tools from the human proteome to facilitate biomedical research. Here we report the generation and multimodal liquid chromatography-tandem mass spectrometry analysis of >330,000 synthetic tryptic peptides representing essentially all canonical human gene products, and we exemplify the utility of these data in several applications. The resource (available at http://www.proteometools.org) will be extended to >1 million peptides, and all data will be shared with the community via ProteomicsDB and ProteomeXchange.

Original language	English
Pages (from-to)	259-262
Number of pages	4
Journal	Nature Methods
Volume	14
Issue number	3
DOIs	https://doi.org/10.1038/nmeth.4153
State	Published - 28 Feb 2017

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Zolg, D. P., Wilhelm, M., Schnatbaum, K., Zerweck, J., Knaute, T., Delanghe, B., Bailey, D. J., Gessulat, S., Ehrlich, H. C., Weininger, M., Yu, P., Schlegl, J., Kramer, K., Schmidt, T., Kusebauch, U., Deutsch, E. W., Aebersold, R., Moritz, R. L., Wenschuh, H., ... Kuster, B. (2017). Building ProteomeTools based on a complete synthetic human proteome. Nature Methods, 14(3), 259-262. https://doi.org/10.1038/nmeth.4153

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abstract = "We describe ProteomeTools, a project building molecular and digital tools from the human proteome to facilitate biomedical research. Here we report the generation and multimodal liquid chromatography-tandem mass spectrometry analysis of >330,000 synthetic tryptic peptides representing essentially all canonical human gene products, and we exemplify the utility of these data in several applications. The resource (available at http://www.proteometools.org) will be extended to >1 million peptides, and all data will be shared with the community via ProteomicsDB and ProteomeXchange.",

author = "Zolg, {Daniel P.} and Mathias Wilhelm and Karsten Schnatbaum and Johannes Zerweck and Tobias Knaute and Bernard Delanghe and Bailey, {Derek J.} and Siegfried Gessulat and Ehrlich, {Hans Christian} and Maximilian Weininger and Peng Yu and Judith Schlegl and Karl Kramer and Tobias Schmidt and Ulrike Kusebauch and Deutsch, {Eric W.} and Ruedi Aebersold and Moritz, {Robert L.} and Holger Wenschuh and Thomas Moehring and Stephan Aiche and Andreas Huhmer and Ulf Reimer and Bernhard Kuster",

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Zolg, DP, Wilhelm, M, Schnatbaum, K, Zerweck, J, Knaute, T, Delanghe, B, Bailey, DJ, Gessulat, S, Ehrlich, HC, Weininger, M, Yu, P, Schlegl, J, Kramer, K, Schmidt, T, Kusebauch, U, Deutsch, EW, Aebersold, R, Moritz, RL, Wenschuh, H, Moehring, T, Aiche, S, Huhmer, A, Reimer, U & Kuster, B 2017, 'Building ProteomeTools based on a complete synthetic human proteome', Nature Methods, vol. 14, no. 3, pp. 259-262. https://doi.org/10.1038/nmeth.4153

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T1 - Building ProteomeTools based on a complete synthetic human proteome

AU - Zolg, Daniel P.

AU - Wilhelm, Mathias

AU - Schnatbaum, Karsten

AU - Zerweck, Johannes

AU - Knaute, Tobias

AU - Delanghe, Bernard

AU - Bailey, Derek J.

AU - Gessulat, Siegfried

AU - Ehrlich, Hans Christian

AU - Weininger, Maximilian

AU - Yu, Peng

AU - Schlegl, Judith

AU - Kramer, Karl

AU - Schmidt, Tobias

AU - Kusebauch, Ulrike

AU - Deutsch, Eric W.

AU - Aebersold, Ruedi

AU - Moritz, Robert L.

AU - Wenschuh, Holger

AU - Moehring, Thomas

AU - Aiche, Stephan

AU - Huhmer, Andreas

AU - Reimer, Ulf

AU - Kuster, Bernhard

PY - 2017/2/28

Y1 - 2017/2/28

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AB - We describe ProteomeTools, a project building molecular and digital tools from the human proteome to facilitate biomedical research. Here we report the generation and multimodal liquid chromatography-tandem mass spectrometry analysis of >330,000 synthetic tryptic peptides representing essentially all canonical human gene products, and we exemplify the utility of these data in several applications. The resource (available at http://www.proteometools.org) will be extended to >1 million peptides, and all data will be shared with the community via ProteomicsDB and ProteomeXchange.

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Building ProteomeTools based on a complete synthetic human proteome

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