Buffered λ-DNA solutions at high shear rates

H. Dakhil, S. K. Basu, S. Steiner, Y. Gerlach, A. Soller, Sharadwata Pan, Natalie Germann, M. Leidenberger, B. Kappes, A. Wierschem

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We study buffered aqueous solutions of deoxyribonucleic acid isolated from bacteriophage lambda (λ-DNA) at shear rates up to 105 s-1. The shear rates are accessed with a narrow-gap rheometer at gap widths down to 20 μm. At lower shear rates, our data merge with the literature values. At high shear rates, the viscosity levels off into an infinite-shear viscosity plateau. Hence, the viscosity functions of buffered aqueous DNA solutions are now available for the entire shear-rate range from the first Newtonian plateau to that of infinite-shear viscosity. The latter hardly differs from the solvent viscosity. For the normal-stress differences, we observe a power-law dependence on the shear rate close to previous findings up to shear rates of about 104 s-1. Beyond this shear-rate range, we observe a stepwise change with the shear rate. By means of agarose gel electrophoresis, we confirm that the λ-DNA is not fragmented during our rheometric study at high shear rates. Yet, at the highest shear rates studied, shear-induced changes in the DNA to structures not being able to travel through the gel appear.

Original languageEnglish
Pages (from-to)159-169
Number of pages11
JournalJournal of Rheology
Volume65
Issue number2
DOIs
StatePublished - 1 Mar 2021

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