TY - JOUR
T1 - Broad-Spectrum Virus Trapping with Heparan Sulfate-Modified DNA Origami Shells
AU - Monferrer, Alba
AU - Kretzmann, Jessica A.
AU - Sigl, Christian
AU - Sapelza, Pia
AU - Liedl, Anna
AU - Wittmann, Barbara
AU - Dietz, Hendrik
N1 - Publisher Copyright:
© 2022 American Chemical Society.
PY - 2022/12/27
Y1 - 2022/12/27
N2 - Effective broadband antiviral platforms that can act on existing viruses and viruses yet to emerge are not available, creating a need to explore treatment strategies beyond the trodden paths. Here, we report virus-encapsulating DNA origami shells that achieve broadband virus trapping properties by exploiting avidity and a widespread background affinity of viruses to heparan sulfate proteoglycans (HSPG). With a calibrated density of heparin and heparan sulfate (HS) derivatives crafted to the interior of DNA origami shells, we could encapsulate adeno, adeno-associated, chikungunya, dengue, human papilloma, noro, polio, rubella, and SARS-CoV-2 viruses or virus-like particles, in one and the same HS-functionalized shell system. Additional virus-type-specific binders were not needed for the trapping. Depending on the relative dimensions of shell to virus particles, multiple virus particles may be trapped per shell, and multiple shells can cover the surface of clusters of virus particles. The steric occlusion provided by the heparan sulfate-coated DNA origami shells can prevent viruses from further interactions with receptors, possibly including those found on cell surfaces.
AB - Effective broadband antiviral platforms that can act on existing viruses and viruses yet to emerge are not available, creating a need to explore treatment strategies beyond the trodden paths. Here, we report virus-encapsulating DNA origami shells that achieve broadband virus trapping properties by exploiting avidity and a widespread background affinity of viruses to heparan sulfate proteoglycans (HSPG). With a calibrated density of heparin and heparan sulfate (HS) derivatives crafted to the interior of DNA origami shells, we could encapsulate adeno, adeno-associated, chikungunya, dengue, human papilloma, noro, polio, rubella, and SARS-CoV-2 viruses or virus-like particles, in one and the same HS-functionalized shell system. Additional virus-type-specific binders were not needed for the trapping. Depending on the relative dimensions of shell to virus particles, multiple virus particles may be trapped per shell, and multiple shells can cover the surface of clusters of virus particles. The steric occlusion provided by the heparan sulfate-coated DNA origami shells can prevent viruses from further interactions with receptors, possibly including those found on cell surfaces.
KW - DNA origami
KW - antiviral
KW - broad-spectrum
KW - heparan sulfate
KW - heparin
KW - virus-like particles
UR - http://www.scopus.com/inward/record.url?scp=85141765113&partnerID=8YFLogxK
U2 - 10.1021/acsnano.1c11328
DO - 10.1021/acsnano.1c11328
M3 - Article
C2 - 36323320
AN - SCOPUS:85141765113
SN - 1936-0851
VL - 16
SP - 20002
EP - 20009
JO - ACS Nano
JF - ACS Nano
IS - 12
ER -