Biosynthesis of the antimetabolite 6-thioguanine in Erwinia amylovora plays a key role in fire blight pathogenesis

Sébastien Coyne; Cornelia Chizzali; Mohammed N.A. Khalil; Agnieszka Litomska; Klaus Richter; Ludger Beerhues; Christian Hertweck

doi:10.1002/anie.201305595

Biosynthesis of the antimetabolite 6-thioguanine in Erwinia amylovora plays a key role in fire blight pathogenesis

Sébastien Coyne, Cornelia Chizzali, Mohammed N.A. Khalil, Agnieszka Litomska, Klaus Richter, Ludger Beerhues, Christian Hertweck

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Sulfur for fire: The molecular basis for the biosynthesis of the antimetabolite 6-thioguanine (6TG) was unveiled in Erwinia amylovora, the causative agent of fire blight. Bioinformatics, heterologous pathway reconstitution in E. coli, and mutational analyses indicate that the protein YcfA mediates guanine thionation in analogy to 2-thiouridylase. Assays in planta and in cell cultures reveal for the first time a crucial role of 6TG in fire blight pathogenesis.

Original language	English
Pages (from-to)	10564-10568
Number of pages	5
Journal	Angewandte Chemie International Edition in English
Volume	52
Issue number	40
DOIs	https://doi.org/10.1002/anie.201305595
State	Published - 27 Sep 2013
Externally published	Yes

Keywords

Erwinia amylovora
antimetabolites
natural products
phytochemistry
sulfur compounds

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10.1002/anie.201305595

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abstract = "Sulfur for fire: The molecular basis for the biosynthesis of the antimetabolite 6-thioguanine (6TG) was unveiled in Erwinia amylovora, the causative agent of fire blight. Bioinformatics, heterologous pathway reconstitution in E. coli, and mutational analyses indicate that the protein YcfA mediates guanine thionation in analogy to 2-thiouridylase. Assays in planta and in cell cultures reveal for the first time a crucial role of 6TG in fire blight pathogenesis.",

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AU - Coyne, Sébastien

AU - Chizzali, Cornelia

AU - Khalil, Mohammed N.A.

AU - Litomska, Agnieszka

AU - Richter, Klaus

AU - Beerhues, Ludger

AU - Hertweck, Christian

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KW - Erwinia amylovora

KW - antimetabolites

KW - natural products

KW - phytochemistry

KW - sulfur compounds

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Biosynthesis of the antimetabolite 6-thioguanine in Erwinia amylovora plays a key role in fire blight pathogenesis

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