Bioreactor design for the mechanical stimulation by compression of 3D cell cultures

Carina M. Micheler; Paulina A. Geck; Fiona Charitou; René Leix; Peter Foehr; Jan J. Lang; Nikolas J. Wilhelm; Jutta L. Tuebel; Rainer H.H. Burgkart

doi:10.1515/cdbme-2021-2229

Bioreactor design for the mechanical stimulation by compression of 3D cell cultures

Carina M. Micheler, Paulina A. Geck, Fiona Charitou, René Leix, Peter Foehr, Jan J. Lang, Nikolas J. Wilhelm, Jutta L. Tuebel, Rainer H.H. Burgkart

Chair of Orthopedics and Sports Orthopedics

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Bioreactors with a controlled physiological environment are being developed to study various cell processes. The influences of mechanostimulation on bone cell cultures can be investigated using a compression bioreactor. The developed bioreactor system applies a cyclic compression force to the specimen via an eccentrically mounted push rod. The compression force is monitored by a force sensor to detect changes in the material properties of the specimen. Depending on the piston setting, a stroke of 0.28 - 2.50 mm can be applied to the specimen. The bioreactor system was tested with a trial run of 18 days. A sample was continuously stimulated with a loading frequency of 2 Hz and a stroke of 1.50 mm.

Original language	English
Pages (from-to)	899-902
Number of pages	4
Journal	Current Directions in Biomedical Engineering
Volume	7
Issue number	2
DOIs	https://doi.org/10.1515/cdbme-2021-2229
State	Published - 1 Oct 2021

Keywords

3D Cell Culture
Bioreactor
Bone Cell Culture
Compression
Osteoblasts
Tissue Engineering

Access to Document

10.1515/cdbme-2021-2229

Cite this

@article{5d82ce694eda4c639387ab1348e68747,

title = "Bioreactor design for the mechanical stimulation by compression of 3D cell cultures",

abstract = "Bioreactors with a controlled physiological environment are being developed to study various cell processes. The influences of mechanostimulation on bone cell cultures can be investigated using a compression bioreactor. The developed bioreactor system applies a cyclic compression force to the specimen via an eccentrically mounted push rod. The compression force is monitored by a force sensor to detect changes in the material properties of the specimen. Depending on the piston setting, a stroke of 0.28 - 2.50 mm can be applied to the specimen. The bioreactor system was tested with a trial run of 18 days. A sample was continuously stimulated with a loading frequency of 2 Hz and a stroke of 1.50 mm.",

keywords = "3D Cell Culture, Bioreactor, Bone Cell Culture, Compression, Osteoblasts, Tissue Engineering",

author = "Micheler, {Carina M.} and Geck, {Paulina A.} and Fiona Charitou and Ren{\'e} Leix and Peter Foehr and Lang, {Jan J.} and Wilhelm, {Nikolas J.} and Tuebel, {Jutta L.} and Burgkart, {Rainer H.H.}",

note = "Publisher Copyright: {\textcopyright} 2021 by Walter de Gruyter Berlin/Boston.",

year = "2021",

month = oct,

day = "1",

doi = "10.1515/cdbme-2021-2229",

language = "English",

volume = "7",

pages = "899--902",

journal = "Current Directions in Biomedical Engineering",

issn = "2364-5504",

publisher = "Walter de Gruyter GmbH",

number = "2",

}

TY - JOUR

T1 - Bioreactor design for the mechanical stimulation by compression of 3D cell cultures

AU - Micheler, Carina M.

AU - Geck, Paulina A.

AU - Charitou, Fiona

AU - Leix, René

AU - Foehr, Peter

AU - Lang, Jan J.

AU - Wilhelm, Nikolas J.

AU - Tuebel, Jutta L.

AU - Burgkart, Rainer H.H.

PY - 2021/10/1

Y1 - 2021/10/1

N2 - Bioreactors with a controlled physiological environment are being developed to study various cell processes. The influences of mechanostimulation on bone cell cultures can be investigated using a compression bioreactor. The developed bioreactor system applies a cyclic compression force to the specimen via an eccentrically mounted push rod. The compression force is monitored by a force sensor to detect changes in the material properties of the specimen. Depending on the piston setting, a stroke of 0.28 - 2.50 mm can be applied to the specimen. The bioreactor system was tested with a trial run of 18 days. A sample was continuously stimulated with a loading frequency of 2 Hz and a stroke of 1.50 mm.

AB - Bioreactors with a controlled physiological environment are being developed to study various cell processes. The influences of mechanostimulation on bone cell cultures can be investigated using a compression bioreactor. The developed bioreactor system applies a cyclic compression force to the specimen via an eccentrically mounted push rod. The compression force is monitored by a force sensor to detect changes in the material properties of the specimen. Depending on the piston setting, a stroke of 0.28 - 2.50 mm can be applied to the specimen. The bioreactor system was tested with a trial run of 18 days. A sample was continuously stimulated with a loading frequency of 2 Hz and a stroke of 1.50 mm.

KW - 3D Cell Culture

KW - Bioreactor

KW - Bone Cell Culture

KW - Compression

KW - Osteoblasts

KW - Tissue Engineering

UR - http://www.scopus.com/inward/record.url?scp=85121916775&partnerID=8YFLogxK

U2 - 10.1515/cdbme-2021-2229

DO - 10.1515/cdbme-2021-2229

M3 - Article

AN - SCOPUS:85121916775

SN - 2364-5504

VL - 7

SP - 899

EP - 902

JO - Current Directions in Biomedical Engineering

JF - Current Directions in Biomedical Engineering

IS - 2

ER -

Bioreactor design for the mechanical stimulation by compression of 3D cell cultures

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this