Biomimetic mineralisation of apatites on Ca2+ activated cellulose templates

Peter Cromme; Cordt Zollfrank; Lenka Müller; Frank A. Müller; Peter Greil

doi:10.1016/j.msec.2005.11.001

Biomimetic mineralisation of apatites on Ca²⁺ activated cellulose templates

Peter Cromme, Cordt Zollfrank, Lenka Müller, Frank A. Müller, Peter Greil

Friedrich Alexander Universität Erlangen-Nürnberg

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

We investigated the activation of regenerated cellulose 2D model thin films and 3D fabric templates with calcium dihydroxide. The Langmuir-Blodgett (LB) film technique was applied for manufacturing of the model thin films using a trimethylsilyl derivative of cellulose (TMS-cellulose). Regenerated cellulose films were obtained by treating the TMS-cellulose LB-films with hydrochloric acid vapours. For 3D templates, regenerated cellulose fabrics (Lyocell®) were used. The regenerated cellulose templates were activated with a Ca(OH)₂-suspension and subsequently exposed to 1.5 × SBF to induce the in situ formation of biomimetic calcium phosphate phases. FTIR and Raman spectroscopy showed that the Ca(OH)₂ and calcite present from reaction with HCO₃^- on the template surface were dissolved in the initial stage of exposure to the 1.5 × SBF. After 1 day, the formation of apatitic phases in 1.5 × SBF was observed. According to detailed calculations, high supersaturation levels S in close vicinity to the template surface (S > 16) resulting from the Ca²⁺ diffusion induced the formation of biomimetic calcium phosphate. The biomimetic calcium phosphates were identified by FTIR and Raman spectroscopy as highly carbonated apatites (HCA) lacking hydroxyl ions. 3D fabric templates of regenerated cellulose covered with a biomimetic coating of apatite might be of particular interest for novel scaffold architectures in bone repair and tissue engineering.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Materials Science and Engineering C
Volume	27
Issue number	1
DOIs	https://doi.org/10.1016/j.msec.2005.11.001
State	Published - Jan 2007
Externally published	Yes

Keywords

Biomimetic mineralisation
Calcium phosphate
Cellulose
Simulated body fluid

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10.1016/j.msec.2005.11.001

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title = "Biomimetic mineralisation of apatites on Ca2+ activated cellulose templates",

abstract = "We investigated the activation of regenerated cellulose 2D model thin films and 3D fabric templates with calcium dihydroxide. The Langmuir-Blodgett (LB) film technique was applied for manufacturing of the model thin films using a trimethylsilyl derivative of cellulose (TMS-cellulose). Regenerated cellulose films were obtained by treating the TMS-cellulose LB-films with hydrochloric acid vapours. For 3D templates, regenerated cellulose fabrics (Lyocell{\textregistered}) were used. The regenerated cellulose templates were activated with a Ca(OH)2-suspension and subsequently exposed to 1.5 × SBF to induce the in situ formation of biomimetic calcium phosphate phases. FTIR and Raman spectroscopy showed that the Ca(OH)2 and calcite present from reaction with HCO3- on the template surface were dissolved in the initial stage of exposure to the 1.5 × SBF. After 1 day, the formation of apatitic phases in 1.5 × SBF was observed. According to detailed calculations, high supersaturation levels S in close vicinity to the template surface (S > 16) resulting from the Ca2+ diffusion induced the formation of biomimetic calcium phosphate. The biomimetic calcium phosphates were identified by FTIR and Raman spectroscopy as highly carbonated apatites (HCA) lacking hydroxyl ions. 3D fabric templates of regenerated cellulose covered with a biomimetic coating of apatite might be of particular interest for novel scaffold architectures in bone repair and tissue engineering.",

keywords = "Biomimetic mineralisation, Calcium phosphate, Cellulose, Simulated body fluid",

author = "Peter Cromme and Cordt Zollfrank and Lenka M{\"u}ller and M{\"u}ller, {Frank A.} and Peter Greil",

note = "Funding Information: Financial support from the German Science Foundation (DFG) and the University of Erlangen-N{\"u}rnberg is gratefully acknowledged. The TEM-work was carried out at the Central Facility for High Resolution Electron Microscopy of the University of Erlangen-N{\"u}rnberg. The authors are grateful to Dietmar Auhl, Institute of Polymer Materials, for the GPC measurements and the Institute of Science and Technology of Metals, both University of Erlangen-N{\"u}rnberg, for the utilisation of the Raman resources.",

year = "2007",

month = jan,

doi = "10.1016/j.msec.2005.11.001",

language = "English",

volume = "27",

pages = "1--7",

journal = "Materials Science and Engineering C",

issn = "0928-4931",

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T1 - Biomimetic mineralisation of apatites on Ca2+ activated cellulose templates

AU - Cromme, Peter

AU - Zollfrank, Cordt

AU - Müller, Lenka

AU - Müller, Frank A.

AU - Greil, Peter

N1 - Funding Information: Financial support from the German Science Foundation (DFG) and the University of Erlangen-Nürnberg is gratefully acknowledged. The TEM-work was carried out at the Central Facility for High Resolution Electron Microscopy of the University of Erlangen-Nürnberg. The authors are grateful to Dietmar Auhl, Institute of Polymer Materials, for the GPC measurements and the Institute of Science and Technology of Metals, both University of Erlangen-Nürnberg, for the utilisation of the Raman resources.

PY - 2007/1

Y1 - 2007/1

N2 - We investigated the activation of regenerated cellulose 2D model thin films and 3D fabric templates with calcium dihydroxide. The Langmuir-Blodgett (LB) film technique was applied for manufacturing of the model thin films using a trimethylsilyl derivative of cellulose (TMS-cellulose). Regenerated cellulose films were obtained by treating the TMS-cellulose LB-films with hydrochloric acid vapours. For 3D templates, regenerated cellulose fabrics (Lyocell®) were used. The regenerated cellulose templates were activated with a Ca(OH)2-suspension and subsequently exposed to 1.5 × SBF to induce the in situ formation of biomimetic calcium phosphate phases. FTIR and Raman spectroscopy showed that the Ca(OH)2 and calcite present from reaction with HCO3- on the template surface were dissolved in the initial stage of exposure to the 1.5 × SBF. After 1 day, the formation of apatitic phases in 1.5 × SBF was observed. According to detailed calculations, high supersaturation levels S in close vicinity to the template surface (S > 16) resulting from the Ca2+ diffusion induced the formation of biomimetic calcium phosphate. The biomimetic calcium phosphates were identified by FTIR and Raman spectroscopy as highly carbonated apatites (HCA) lacking hydroxyl ions. 3D fabric templates of regenerated cellulose covered with a biomimetic coating of apatite might be of particular interest for novel scaffold architectures in bone repair and tissue engineering.

AB - We investigated the activation of regenerated cellulose 2D model thin films and 3D fabric templates with calcium dihydroxide. The Langmuir-Blodgett (LB) film technique was applied for manufacturing of the model thin films using a trimethylsilyl derivative of cellulose (TMS-cellulose). Regenerated cellulose films were obtained by treating the TMS-cellulose LB-films with hydrochloric acid vapours. For 3D templates, regenerated cellulose fabrics (Lyocell®) were used. The regenerated cellulose templates were activated with a Ca(OH)2-suspension and subsequently exposed to 1.5 × SBF to induce the in situ formation of biomimetic calcium phosphate phases. FTIR and Raman spectroscopy showed that the Ca(OH)2 and calcite present from reaction with HCO3- on the template surface were dissolved in the initial stage of exposure to the 1.5 × SBF. After 1 day, the formation of apatitic phases in 1.5 × SBF was observed. According to detailed calculations, high supersaturation levels S in close vicinity to the template surface (S > 16) resulting from the Ca2+ diffusion induced the formation of biomimetic calcium phosphate. The biomimetic calcium phosphates were identified by FTIR and Raman spectroscopy as highly carbonated apatites (HCA) lacking hydroxyl ions. 3D fabric templates of regenerated cellulose covered with a biomimetic coating of apatite might be of particular interest for novel scaffold architectures in bone repair and tissue engineering.

KW - Biomimetic mineralisation

KW - Calcium phosphate

KW - Cellulose

KW - Simulated body fluid

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DO - 10.1016/j.msec.2005.11.001

M3 - Article

AN - SCOPUS:33751538330

SN - 0928-4931

VL - 27

SP - 1

EP - 7

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

IS - 1

ER -

Biomimetic mineralisation of apatites on Ca²⁺ activated cellulose templates

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Keywords

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