@inbook{dd59356f4bf54d05a2311e5a873005ca,
title = "Bioengineered bacterial vesicles for optoacoustics-guided phototherapy",
abstract = "Genetically engineered bacterial outer membrane vesicles (OMVs) offer promising applications for gene therapy, immunotherapy, and vaccine delivery. Importantly, OMVs are biocompatible, biodegradable, and easy to engineer and produce on a large scale. In this chapter, we discuss the development and application of bioengineered OMVs for optoacoustics-guided phototherapy applications (theranostics). We provide detailed protocols for OMVs preparation, characterization, and in vitro and in vivo validation. The engineered OMVs carry the biopolymer melanin, which generates a strong optoacoustic (OA) signal and intense heat upon absorption of near-infrared (NIR) light, enabling optoacoustics-guided cancer diagnosis and photothermal therapy in vivo.",
keywords = "Bacterial outer membrane vesicles, Cancer, Melanin, Optoacoustics, Phototherapy, Theranostics",
author = "Vipul Gujrati and Vasilis Ntziachristos",
note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = jan,
doi = "10.1016/bs.mie.2021.06.030",
language = "English",
isbn = "9780323855303",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "349--364",
editor = "Jefferson Chan",
booktitle = "Photoacoustic Probes for In Vivo Imaging",
}
