TY - GEN
T1 - Biocompatibility assessment of Sic surfaces after functionalization with self assembled organic monolayers
AU - Oliveros, Alexandra
AU - Schoell, Sebastian J.
AU - Frewin, Christopher
AU - Hoeb, Marco
AU - Stutzmann, Martin
AU - Sharp, Ian D.
AU - Saddow, Stephen E.
PY - 2010
Y1 - 2010
N2 - The biocompatibility of 6H-SiC (0001) surfaces was increased by more than a factor of six through the covalent grafting of NH2 terminated self-assembled monolayers (SAM) using APDEMS and APTES molecules. Surface functionalization began with a hydroxyl, OH, surface termination. The study included two NH2 terminated surfaces obtained through silanization with APDEMS (aminopropyldiethoxymethylsilane) and APTES (aminopropyltriethoxysilane) molecules (hydrophilic surfaces) and a CH 3 terminated surface produced via alkylation with 1-octadecene (hydrophobic surface). H4 human neuroglioma and PC12 rat pheochromocytoma cells were seeded on the functionalized surfaces and the cell morphology was evaluated with atomic force microscopy (AFM). In addition, 96 hour MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays were employed to evaluate the cell viability on the SAM modified samples. The biocompatibility was enhanced with a 2 fold (171-240%) increase with 1-octadecene, 3-6 fold (320-670%) increase with APDEMS and 5-8 fold (476-850%) increase with APTES with respect to untreated 6H-SiC surfaces.
AB - The biocompatibility of 6H-SiC (0001) surfaces was increased by more than a factor of six through the covalent grafting of NH2 terminated self-assembled monolayers (SAM) using APDEMS and APTES molecules. Surface functionalization began with a hydroxyl, OH, surface termination. The study included two NH2 terminated surfaces obtained through silanization with APDEMS (aminopropyldiethoxymethylsilane) and APTES (aminopropyltriethoxysilane) molecules (hydrophilic surfaces) and a CH 3 terminated surface produced via alkylation with 1-octadecene (hydrophobic surface). H4 human neuroglioma and PC12 rat pheochromocytoma cells were seeded on the functionalized surfaces and the cell morphology was evaluated with atomic force microscopy (AFM). In addition, 96 hour MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays were employed to evaluate the cell viability on the SAM modified samples. The biocompatibility was enhanced with a 2 fold (171-240%) increase with 1-octadecene, 3-6 fold (320-670%) increase with APDEMS and 5-8 fold (476-850%) increase with APTES with respect to untreated 6H-SiC surfaces.
UR - http://www.scopus.com/inward/record.url?scp=77957757111&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:77957757111
SN - 9781617387654
T3 - Materials Research Society Symposium Proceedings
SP - 13
EP - 18
BT - Engineering Biomaterials for Regenerative Medicine
T2 - 2009 MRS Fall Meeting
Y2 - 30 November 2009 through 4 December 2009
ER -