Biochemical activities of extracts from Hypericum perforatum L. / 3rd Communication: Modulation of peroxidase activity as a simple method for standardization

Harald Schempp, Andrea Denke, Elke Mann, Werner Schneider, Erich F. Elstner

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Alcoholic extracts from the herb 'St. John's wort' (Hypericum perforatum L) are widely used to counteract depressive situations, where the question on the mainly active principle is still under discussion. Thus, standardization of the drug on the basis of dry matter has been chosen instead of the popular leading component, hypericin. Inhibition of myeloperoxidase-catalyzed dimerization of enkephalins by Hypericum extracts has recently been reported. This method is based on the separation and quantification of enkephalin dimers by HPLC. In order to simplify this assay myeloperoxidase could be substituted by the cheaper horseradish peroxidase and the enkephalins by the amino acid tyrosine without loss of significance. In this communication we represent a more rapid photometric method based on peroxidase-catalyzed indole acetic acid oxidation suitable for quick, simple and economic drug standardization.

Original languageEnglish
Pages (from-to)115-119
Number of pages5
JournalArzneimittel-Forschung/Drug Research
Volume49
Issue number2
DOIs
StatePublished - 1999

Keywords

  • Antidepressants
  • Horseradish peroxidase
  • Hypericum perforatum L. extracts
  • Indole acetic acid oxidation

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