Bioassay zur bestimmung von TCDD-toxizitatsaquivalenten (TEQ) von umweltproben und reststoffen

A. M. Hofmaier; S. M.G. Schwirzer; F. J. Wiebel; K. W. Schramm; M. Wegenke; A. A.F. Kettrup

doi:10.1007/bf03037752

Bioassay zur bestimmung von TCDD-toxizitatsaquivalenten (TEQ) von umweltproben und reststoffen

Translated title of the contribution: A bioassay for determination of TCDD-toxicity equivalents (TEQ) of environmental samples and residual wastes

A. M. Hofmaier
, S. M.G. Schwirzer
, F. J. Wiebel
, K. W. Schramm
, M. Wegenke
, A. A.F. Kettrup

Life Sciences

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Polychlorinated aromatic hydrocarbons (HAH) like 2,3,7,8-tetrachloro-p- dioxin (TCDD) elicit a multitude of toxic and biological effects. A common trait of these substances is their affinity for a cytosolic receptor protein and the subsequent induction of the synthesis of several gene products, including cytochrome P450 1A1 (CYP 1A1). In this publication we present a bioassay to determine the induction of CYP 1A1 as a sum parameter for the total toxic potential of critical halogenated compounds in complex environmental matrices. In order to eliminate compounds like polycylic aromatic hydrocarbons (PAH) and naturally occurring substances which might interfere with the bioassay we developed a simple cleanup procedure. TEQ values derived from bioassay are in good agreement with those obtained from chemical analysis. This bioassay in combination with the simplified 'single column' clean up represents a cost and time effective alternative to chemical analysis for the screening of large numbers of environmental matrices.

Translated title of the contribution	A bioassay for determination of TCDD-toxicity equivalents (TEQ) of environmental samples and residual wastes
Original language	German
Pages (from-to)	2-8
Number of pages	7
Journal	Umweltwissenschaften und Schadstoff-Forschung
Volume	11
Issue number	1
DOIs	https://doi.org/10.1007/bf03037752
State	Published - 1999

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title = "Bioassay zur bestimmung von TCDD-toxizitatsaquivalenten (TEQ) von umweltproben und reststoffen",

abstract = "Polychlorinated aromatic hydrocarbons (HAH) like 2,3,7,8-tetrachloro-p- dioxin (TCDD) elicit a multitude of toxic and biological effects. A common trait of these substances is their affinity for a cytosolic receptor protein and the subsequent induction of the synthesis of several gene products, including cytochrome P450 1A1 (CYP 1A1). In this publication we present a bioassay to determine the induction of CYP 1A1 as a sum parameter for the total toxic potential of critical halogenated compounds in complex environmental matrices. In order to eliminate compounds like polycylic aromatic hydrocarbons (PAH) and naturally occurring substances which might interfere with the bioassay we developed a simple cleanup procedure. TEQ values derived from bioassay are in good agreement with those obtained from chemical analysis. This bioassay in combination with the simplified 'single column' clean up represents a cost and time effective alternative to chemical analysis for the screening of large numbers of environmental matrices.",

keywords = "Bioassay, Cytochrom P450 1A1, H4IIE-cells, PHAK, Tetrachlordibenzo-p-dioxin (TCDD), Toxicity equivalents (TEQ)",

author = "Hofmaier, \{A. M.\} and Schwirzer, \{S. M.G.\} and Wiebel, \{F. J.\} and Schramm, \{K. W.\} and M. Wegenke and Kettrup, \{A. A.F.\}",

year = "1999",

doi = "10.1007/bf03037752",

language = "Deutsch",

volume = "11",

pages = "2--8",

journal = "Umweltwissenschaften und Schadstoff-Forschung",

issn = "0934-3504",

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number = "1",

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T1 - Bioassay zur bestimmung von TCDD-toxizitatsaquivalenten (TEQ) von umweltproben und reststoffen

AU - Hofmaier, A. M.

AU - Schwirzer, S. M.G.

AU - Wiebel, F. J.

AU - Schramm, K. W.

AU - Wegenke, M.

AU - Kettrup, A. A.F.

PY - 1999

Y1 - 1999

N2 - Polychlorinated aromatic hydrocarbons (HAH) like 2,3,7,8-tetrachloro-p- dioxin (TCDD) elicit a multitude of toxic and biological effects. A common trait of these substances is their affinity for a cytosolic receptor protein and the subsequent induction of the synthesis of several gene products, including cytochrome P450 1A1 (CYP 1A1). In this publication we present a bioassay to determine the induction of CYP 1A1 as a sum parameter for the total toxic potential of critical halogenated compounds in complex environmental matrices. In order to eliminate compounds like polycylic aromatic hydrocarbons (PAH) and naturally occurring substances which might interfere with the bioassay we developed a simple cleanup procedure. TEQ values derived from bioassay are in good agreement with those obtained from chemical analysis. This bioassay in combination with the simplified 'single column' clean up represents a cost and time effective alternative to chemical analysis for the screening of large numbers of environmental matrices.

AB - Polychlorinated aromatic hydrocarbons (HAH) like 2,3,7,8-tetrachloro-p- dioxin (TCDD) elicit a multitude of toxic and biological effects. A common trait of these substances is their affinity for a cytosolic receptor protein and the subsequent induction of the synthesis of several gene products, including cytochrome P450 1A1 (CYP 1A1). In this publication we present a bioassay to determine the induction of CYP 1A1 as a sum parameter for the total toxic potential of critical halogenated compounds in complex environmental matrices. In order to eliminate compounds like polycylic aromatic hydrocarbons (PAH) and naturally occurring substances which might interfere with the bioassay we developed a simple cleanup procedure. TEQ values derived from bioassay are in good agreement with those obtained from chemical analysis. This bioassay in combination with the simplified 'single column' clean up represents a cost and time effective alternative to chemical analysis for the screening of large numbers of environmental matrices.

KW - Bioassay

KW - Cytochrom P450 1A1

KW - H4IIE-cells

KW - PHAK

KW - Tetrachlordibenzo-p-dioxin (TCDD)

KW - Toxicity equivalents (TEQ)

UR - https://www.scopus.com/pages/publications/0032888344

U2 - 10.1007/bf03037752

DO - 10.1007/bf03037752

M3 - Artikel

AN - SCOPUS:0032888344

SN - 0934-3504

VL - 11

SP - 2

EP - 8

JO - Umweltwissenschaften und Schadstoff-Forschung

JF - Umweltwissenschaften und Schadstoff-Forschung

IS - 1

ER -

Bioassay zur bestimmung von TCDD-toxizitatsaquivalenten (TEQ) von umweltproben und reststoffen

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