Bio-inspired in situ crosslinking and mineralization of electrospun collagen scaffolds for bone tissue engineering

Chetna Dhand, Seow Theng Ong, Neeraj Dwivedi, Silvia Marrero Diaz, Jayarama Reddy Venugopal, Balchandar Navaneethan, Mobashar H.U.T. Fazil, Shouping Liu, Vera Seitz, Erich Wintermantel, Roger W. Beuerman, Seeram Ramakrishna, Navin K. Verma, Rajamani Lakshminarayanan

Research output: Contribution to journalArticlepeer-review

163 Scopus citations

Abstract

Bone disorders are the most common cause of severe long term pain and physical disability, and affect millions of people around the world. In the present study, we report bio-inspired preparation of bone-like composite structures by electrospinning of collagen containing catecholamines and Ca2+. The presence of divalent cation induces simultaneous partial oxidative polymerization of catecholamines and crosslinking of collagen nanofibers, thus producing mats that are mechanically robust and confer photoluminescence properties. Subsequent mineralization of the mats by ammonium carbonate leads to complete oxidative polymerization of catecholamines and precipitation of amorphous CaCO3. The collagen composite scaffolds display outstanding mechanical properties with Young's modulus approaching the limits of cancellous bone. Biological studies demonstrate that human fetal osteoblasts seeded on to the composite scaffolds display enhanced cell adhesion, penetration, proliferation, differentiation and osteogenic expression of osteocalcin, osteopontin and bone matrix protein when compared to pristine collagen or tissue culture plates. Among the two catecholamines, mats containing norepinephrine displayed superior mechanical, photoluminescence and biological properties than mats loaded with dopamine. These smart multifunctional scaffolds could potentially be utilized to repair and regenerate bone defects and injuries.

Original languageEnglish
Pages (from-to)323-338
Number of pages16
JournalBiomaterials
Volume104
DOIs
StatePublished - 1 Oct 2016

Keywords

  • Bone
  • Catecholamine
  • Collagen
  • Crosslinking
  • Electrospinning
  • Mineralization

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