Binding and pK a modulation of a polycyclic substrate analogue in a type II polyketide acyl carrier protein

Robert W. Haushalter, Fabian V. Filipp, Kwang Seuk Ko, Ricky Yu, Stanley J. Opella, Michael D. Burkart

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Type II polyketide synthases are biosynthetic enzymatic pathways responsible for the production of complex aromatic natural products with important biological activities. In these systems, biosynthetic intermediates are covalently bound to a small acyl carrier protein that associates with the synthase enzymes and delivers the bound intermediate to each active site. In the closely related fatty acid synthases of bacteria and plants, the acyl carrier protein acts to sequester and protect attached intermediates within its helices. Here we investigate the type II polyketide synthase acyl carrier protein from the actinorhodin biosynthetic pathway and demonstrate its ability to internalize the tricyclic, polar molecule emodic acid. Elucidating the interaction of acyl carrier proteins with bound analogues resembling late-stage intermediates in the actinorhodin pathway could prove valuable in efforts to engineer these systems toward rational design and biosynthesis of novel compounds.

Original languageEnglish
Pages (from-to)413-418
Number of pages6
JournalACS Chemical Biology
Volume6
Issue number5
DOIs
StatePublished - 20 May 2011
Externally publishedYes

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