Baf60c is a nuclear Notch signaling component required for the establishment of left-right asymmetry

Jun K. Takeuchi; Heiko Lickert; Brent W. Bisgrove; Xin Sun; Masamichi Yamamoto; Kallayanee Chawengsaksophak; Hiroshi Hamada; H. Joseph Yost; Janet Rossant; Benoit G. Bruneau

doi:10.1073/pnas.0608118104

Baf60c is a nuclear Notch signaling component required for the establishment of left-right asymmetry

Jun K. Takeuchi, Heiko Lickert, Brent W. Bisgrove, Xin Sun, Masamichi Yamamoto, Kallayanee Chawengsaksophak, Hiroshi Hamada, H. Joseph Yost, Janet Rossant, Benoit G. Bruneau

Research output: Contribution to journal › Article › peer-review

102 Scopus citations

Abstract

Notch-mediated induction of Nodal at the vertebrate node is a critical step in initiating left-right (LR) asymmetry. In mice and zebrafish we show that Baf60c, a subunit of the Swi/Snf-like BAF chromatin remodeling complex, is essential for establishment of LR asymmetry. Baf60c knockdown mouse embryos fail to activate Nodal at the node and also have abnormal node morphology with mixing of crown and pit cells. In cell culture, Baf60c is required for Notch-dependent transcriptional activation and functions to stabilize interactions between activated Notch and its DNA-binding partner, RBP-J. Brg1 is also required for these processes, suggesting that BAF complexes are key components of nuclear Notch signaling. We propose a critical role for Baf60c in Notch-dependent transcription and LR asymmetry.

Original language	English
Pages (from-to)	846-851
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	104
Issue number	3
DOIs	https://doi.org/10.1073/pnas.0608118104
State	Published - 16 Jan 2007
Externally published	Yes

Keywords

Chromatin
Node
Swi/Snf

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10.1073/pnas.0608118104

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Takeuchi, J. K., Lickert, H., Bisgrove, B. W., Sun, X., Yamamoto, M., Chawengsaksophak, K., Hamada, H., Yost, H. J., Rossant, J., & Bruneau, B. G. (2007). Baf60c is a nuclear Notch signaling component required for the establishment of left-right asymmetry. Proceedings of the National Academy of Sciences of the United States of America, 104(3), 846-851. https://doi.org/10.1073/pnas.0608118104

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abstract = "Notch-mediated induction of Nodal at the vertebrate node is a critical step in initiating left-right (LR) asymmetry. In mice and zebrafish we show that Baf60c, a subunit of the Swi/Snf-like BAF chromatin remodeling complex, is essential for establishment of LR asymmetry. Baf60c knockdown mouse embryos fail to activate Nodal at the node and also have abnormal node morphology with mixing of crown and pit cells. In cell culture, Baf60c is required for Notch-dependent transcriptional activation and functions to stabilize interactions between activated Notch and its DNA-binding partner, RBP-J. Brg1 is also required for these processes, suggesting that BAF complexes are key components of nuclear Notch signaling. We propose a critical role for Baf60c in Notch-dependent transcription and LR asymmetry.",

keywords = "Chromatin, Node, Swi/Snf",

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Takeuchi, JK, Lickert, H, Bisgrove, BW, Sun, X, Yamamoto, M, Chawengsaksophak, K, Hamada, H, Yost, HJ, Rossant, J & Bruneau, BG 2007, 'Baf60c is a nuclear Notch signaling component required for the establishment of left-right asymmetry', Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 3, pp. 846-851. https://doi.org/10.1073/pnas.0608118104

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T1 - Baf60c is a nuclear Notch signaling component required for the establishment of left-right asymmetry

AU - Takeuchi, Jun K.

AU - Lickert, Heiko

AU - Bisgrove, Brent W.

AU - Sun, Xin

AU - Yamamoto, Masamichi

AU - Chawengsaksophak, Kallayanee

AU - Hamada, Hiroshi

AU - Yost, H. Joseph

AU - Rossant, Janet

AU - Bruneau, Benoit G.

PY - 2007/1/16

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N2 - Notch-mediated induction of Nodal at the vertebrate node is a critical step in initiating left-right (LR) asymmetry. In mice and zebrafish we show that Baf60c, a subunit of the Swi/Snf-like BAF chromatin remodeling complex, is essential for establishment of LR asymmetry. Baf60c knockdown mouse embryos fail to activate Nodal at the node and also have abnormal node morphology with mixing of crown and pit cells. In cell culture, Baf60c is required for Notch-dependent transcriptional activation and functions to stabilize interactions between activated Notch and its DNA-binding partner, RBP-J. Brg1 is also required for these processes, suggesting that BAF complexes are key components of nuclear Notch signaling. We propose a critical role for Baf60c in Notch-dependent transcription and LR asymmetry.

AB - Notch-mediated induction of Nodal at the vertebrate node is a critical step in initiating left-right (LR) asymmetry. In mice and zebrafish we show that Baf60c, a subunit of the Swi/Snf-like BAF chromatin remodeling complex, is essential for establishment of LR asymmetry. Baf60c knockdown mouse embryos fail to activate Nodal at the node and also have abnormal node morphology with mixing of crown and pit cells. In cell culture, Baf60c is required for Notch-dependent transcriptional activation and functions to stabilize interactions between activated Notch and its DNA-binding partner, RBP-J. Brg1 is also required for these processes, suggesting that BAF complexes are key components of nuclear Notch signaling. We propose a critical role for Baf60c in Notch-dependent transcription and LR asymmetry.

KW - Chromatin

KW - Node

KW - Swi/Snf

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JF - Proceedings of the National Academy of Sciences of the United States of America

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Baf60c is a nuclear Notch signaling component required for the establishment of left-right asymmetry

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