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Bacterial turgor pressure can be measured by atomic force microscopy

  • Markus Arnoldi
  • , Monika Fritz
  • , Edmund Bäuerlein
  • , Manfred Radmacher
  • , Erich Sackmann
  • , Alexei Boulbitch
  • Technical University of Munich
  • Max Planck Institute of Biochemistry
  • University of Munich

Research output: Contribution to journalArticlepeer-review

252 Scopus citations

Abstract

The deformability of a bacterial wall of the gram-negative bacterium Magnetospirillum gryphiswaldense is investigated with an atomic force microscopy (AFM). A theoretical expression is derived for the force exerted by the wall on the cantilever as a function of the depths of indentation generated by the AFM tip. This reaction force is a measure for the turgor pressure of the bacterium. Results determined the turgor pressure to be in the range of 85 to 150 kPa.

Original languageEnglish
Pages (from-to)1034-1044
Number of pages11
JournalPhysical Review E - Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics
Volume62
Issue number1 B
DOIs
StatePublished - Jul 2000

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