Bacterial turgor pressure can be measured by atomic force microscopy

Markus Arnoldi, Monika Fritz, Edmund Bäuerlein, Manfred Radmacher, Erich Sackmann, Alexei Boulbitch

Research output: Contribution to journalArticlepeer-review

247 Scopus citations

Abstract

The deformability of a bacterial wall of the gram-negative bacterium Magnetospirillum gryphiswaldense is investigated with an atomic force microscopy (AFM). A theoretical expression is derived for the force exerted by the wall on the cantilever as a function of the depths of indentation generated by the AFM tip. This reaction force is a measure for the turgor pressure of the bacterium. Results determined the turgor pressure to be in the range of 85 to 150 kPa.

Original languageEnglish
Pages (from-to)1034-1044
Number of pages11
JournalPhysical Review E - Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics
Volume62
Issue number1 B
DOIs
StatePublished - Jul 2000

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