TY - JOUR
T1 - Bacterial glycosyltransferases
T2 - Challenges and Opportunities of a Highly Diverse Enzyme Class Toward Tailoring Natural Products
AU - Schmid, Jochen
AU - Heider, Dominik
AU - Wendel, Norma J.
AU - Sperl, Nadine
AU - Sieber, Volker
N1 - Publisher Copyright:
© 2016 Schmid, Heider, Wendel, Sperl and Sieber.
PY - 2016/2/18
Y1 - 2016/2/18
N2 - The enzyme subclass of glycosyltransferases (GTs; EC 2.4) currently comprises 97 families as specified by CAZy classification. One of their important roles is in the biosynthesis of disaccharides, oligosaccharides, and polysaccharides by catalyzing the transfer of sugar moieties from activated donor molecules to other sugar molecules. In addition GTs also catalyze the transfer of sugar moieties onto aglycons, which is of great relevance for the synthesis of many high value natural products. Bacterial GTs show a higher sequence similarity in comparison to mammalian ones. Even when most GTs are poorly explored, state of the art technologies, such as protein engineering, domain swapping or computational analysis strongly enhance our understanding and utilization of these very promising classes of proteins. This perspective article will focus on bacterial GTs, especially on classification, screening and engineering strategies to alter substrate specificity. The future development in these fields as well as obstacles and challenges will be highlighted and discussed.
AB - The enzyme subclass of glycosyltransferases (GTs; EC 2.4) currently comprises 97 families as specified by CAZy classification. One of their important roles is in the biosynthesis of disaccharides, oligosaccharides, and polysaccharides by catalyzing the transfer of sugar moieties from activated donor molecules to other sugar molecules. In addition GTs also catalyze the transfer of sugar moieties onto aglycons, which is of great relevance for the synthesis of many high value natural products. Bacterial GTs show a higher sequence similarity in comparison to mammalian ones. Even when most GTs are poorly explored, state of the art technologies, such as protein engineering, domain swapping or computational analysis strongly enhance our understanding and utilization of these very promising classes of proteins. This perspective article will focus on bacterial GTs, especially on classification, screening and engineering strategies to alter substrate specificity. The future development in these fields as well as obstacles and challenges will be highlighted and discussed.
KW - Bacterial glycosyltransferases
KW - Categorization of glycosyltransferases
KW - Docking experiments
KW - Polysaccharide glycosyltransferases
KW - Screening
KW - Substrate specificity
UR - http://www.scopus.com/inward/record.url?scp=84962159528&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2016.00182
DO - 10.3389/fmicb.2016.00182
M3 - Article
AN - SCOPUS:84962159528
SN - 1664-302X
VL - 7
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
IS - FEB
M1 - 182
ER -