Bacterial expression of immunoglobulin fragments

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133 Scopus citations

Abstract

The expression of Ig fragments in Escherichia coli permits rapid access to engineered molecules with antigen-binding properties. While the expression in a functional state by secretion to the periplasm is the standard method for the production of Fv and Fab fragments, single chain Fv fragments are mainly produced by refolding from insoluble aggregates. Although all of these Ig fragments serve as valuable aids in the study of antigen binding, their different biochemical properties must be considered when using them as research tools or for medical applications. In addition to these simple univalent antibody fragments, the bacterial expression of bivalent and bispecific versions and of hybrid proteins with novel effector functions is gaining increasing importance.

Original languageEnglish
Pages (from-to)256-262
Number of pages7
JournalCurrent Opinion in Immunology
Volume5
Issue number2
DOIs
StatePublished - 1993
Externally publishedYes

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